PSMD4 Polyklonaler Antikörper

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• KD/KO Validated

PSMD4 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 14899-1-AP

Synonyme

AF, AF 1, Antisecretory factor 1, ASF, MCB1, PSMD4, pUB R5, Rpn10, S5A

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Galerie der Validierungsdaten

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  WB analysis using 14899-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14899-1-AP (PSMD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 14899-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14899-1-AP (PSMD4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14899-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14899-1-AP (PSMD4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 14899-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 14899-1-AP (PSMD4 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 14899-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14899-1-AP (PSMD4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human lung cancer using 14899-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14899-1-AP (PSMD4 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 14899-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 14899-1-AP (PSMD4 Antibody) at dilution of 1:50.

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  IHC staining of human testis using 14899-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 14899-1-AP (PSMD4 Antibody) at dilution of 1:50.

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  IF Staining of HepG2 using 14899-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PSMD4 antibody (14899-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of HepG2 using 14899-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug PSMD4 antibody (14899-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, K-562-Zellen, NIH/3T3-Zellen, Raji-Zellen, Rattenmilzgewebe, RAW264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

14899-1-AP bindet in WB, IHC, IF, FC, ELISA PSMD4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSMD4 fusion protein Ag6691
• Vollständiger Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 4
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC002365
• Gene symbol: PSMD4
• Gene ID (NCBI): 5710
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSMD4, also named as MCB1, AF or ASF, belongs to the proteasome subunit S5A family. PSMD4 is a 50-kD subunit of the regulatory complex of the 26S proteasome. It binds and presumably selects ubiquitin-conjugates for destruction. PSMD4 displays selectivity for longer polyubiquitin chains. It modulates intestinal fluid secretion. PSMD4 is exposed on the sperm acrosomal surface, a notion that was further supported by the binding of non-cell permeant, biotinylated proteasomal inhibitor ZL3VS to the sperm acrosome. It is involved in the sperm-ZP penetration during fertilization. (PMID: 20526895) This antibody is specific to PSMD4.

Publikationen

Species	Application	Title
mouse	WB,IF	Nat Neurosci; Loss of nuclear UBE3A causes electrophysiological and behavioral deficits in mice and is associated with Angelman syndrome.; Authors - Rossella Avagliano Trezza
mouse	IF	J Cell Biol; The transition zone protein Rpgrip1l regulates proteasomal activity at the primary cilium.; Authors - Christoph Gerhardt
human	WB	Cancer Res; The Ubiquitin-like Protein FAT10 Stabilizes eEF1A1 Expression to Promote Tumor Proliferation in a Complex Manner.; Authors - Xiuxia Liu
human	WB	Oncogene; The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a.; Authors - Sparks A A
human	WB	J Cell Sci; S5a binds to death receptor-6 to induce THP-1 monocytes to differentiate through the activation of the NF-κB pathway.; Authors - Zheng Wang
human	WB	Mol Cell Proteomics; Proteaphagy in Mammalian Cells Can Function Independent of ATG5/ATG7.; Authors - Tatjana Goebel

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