PSMD9 Monoklonaler Antikörper

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• KD/KO Validated

PSMD9 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

1H2G1

Kat-Nr. : 67338-1-Ig

Synonyme

p27, PSMD9, Rpn4

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Galerie der Validierungsdaten

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  WB analysis using 67338-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67338-1-Ig (PSMD9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 67338-1-Ig

  WB result of PSMD9 antibody (67338-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMD9 transfected A549 cells.

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  WB analysis using 67338-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67338-1-Ig (PSMD9 antibody) at dilution of 1:2800 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 67338-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67338-1-Ig (PSMD9 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 67338-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67338-1-Ig (PSMD9 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 67338-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using PSMD9 antibody (67338-1-Ig, Clone: 1H2G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HeLa using 67338-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PSMD9 antibody (67338-1-Ig, Clone: 1H2G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, 4T1-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: U2OS-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

67338-1-Ig bindet in WB, IHC, IF, ELISA PSMD9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PSMD9 fusion protein Ag25654
• Vollständiger Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 9
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC004213
• Gene symbol: PSMD9
• Gene ID (NCBI): 5715
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSMD9 is a ubiquitous protein of eukaryotic cells and is a chaperon of the 26S proteasome complex, which degrades ubiquitinated proteins in eukaryotic cells and contributes to the degradation of intracellular proteins into antigenic peptides for antigen presentation by MHC class I cells. The 26S mammalian base sub-complex involves three distinct modules which have ATPase subunits distinctly associated to three chaperones, one of which is PSMD9 regulating the modules assembly. The PSMD9 ubiquitous regulatory role within the proteasome implies its potential pleiotropic effects within different physio-pathological systems. PSMD9 is known to form a stable subcomplex with PSMC3 and PSMC6, two of the AAA-ATPases, assisting in the assembly of the 20S and 19S particles to form the holo complex.

Publikationen

Species	Application	Title
human	WB,IHC,IF	CNS Neurosci Ther; Comprehensive analysis of PSMD family members and validation of PSMD9 as a potential therapeutic target in human glioblastoma; Authors - Yaquan Li