PTEN Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PTEN Polyklonal Antikörper für FC, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

152

Kat-Nr. : 22034-1-AP

Synonyme

10q23del, Bannayan Zonana syndrome, BC005821, BZS, ITGA 2, MGC11227, MHAM, MMAC1, Multiple hamartoma (Cowden syndrome), phosphatase and tensin homolog, PTEN, PTEN1, TEP1

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Galerie der Validierungsdaten

• 

  WB analysis using 22034-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22034-1-AP (PTEN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 22034-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 22034-1-AP (PTEN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 22034-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22034-1-AP (PTEN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 22034-1-PBS

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 22034-1-AP (PTEN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22034-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22034-1-AP (PTEN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22034-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22034-1-AP (PTEN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of DU 145 using 22034-1-AP

  IP result of anti-PTEN (IP:22034-1-AP, 4ug; Detection:22034-1-AP 1:500) with DU 145 cells lysate 1800 ug.

• 

  IHC staining of human breast cancer using 22034-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22034-1-AP (PTEN antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 22034-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22034-1-AP (PTEN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 22034-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22034-1-AP (PTEN Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 22034-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22034-1-AP (PTEN Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 22034-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22034-1-AP (PTEN Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 22034-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22034-1-AP (PTEN Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 22034-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22034-1-AP (PTEN Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 22034-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22034-1-AP (PTEN Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse brain using 22034-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22034-1-AP (PTEN Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse brain using 22034-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22034-1-AP (PTEN Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 22034-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22034-1-AP (PTEN Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 22034-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22034-1-AP (PTEN Antibody) at dilution of 1:200 (under 40x lens).

• 

  FC experiment of HeLa using 22034-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human PTEN (22034-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DU 145-Zellen, C6-Zellen, HEK-293T-Zellen, HeLa-Zellen, Jurkat-Zellen, LNCaP-Zellen, Maushodengewebe, Maus-Thymusgewebe, MCF-7-Zellen, NIH/3T3-Zellen, Rattenhodengewebe
• Erfolgreiche IP: DU 145-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Hodengewebe, humanes Kolonkarzinomgewebe, humanes Prostatakarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 17 publications below
• WB: See 138 publications below
• IHC: See 29 publications below
• IF: See 14 publications below
• IP: See 6 publications below
• CoIP: See 2 publications below

Produktinformation

22034-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA PTEN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PTEN fusion protein Ag17274
• Vollständiger Name: phosphatase and tensin homolog
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 55 kDa, 68 kDa
• GenBank-Zugangsnummer: BC005821
• Gene symbol: PTEN
• Gene ID (NCBI): 5728
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Phosphatase and tensin homolog (PTEN) is an enzyme key in cell cycle regulation and in tumor suppression.

What is the molecular weight of PTEN?

PTEN is composed of 403 amino acid residues with an N-terminal domain with phosphatase activity and a 

C-terminal C2 domain that binds phospholipid membranes (PMID:14749127). This antibody can recognize 

two isoforms with MWs of 55kDa and 68 kDa.

What is the function of PTEN?

Widely expressed throughout the body, PTEN is found in the cytoplasm and inhibits cell cycle progression 

through phosphatase activity. It regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate (PIP3) 

by catalyzing the dephosphorylation of the inositol ring. This function counteracts the growth-stimulating 

activity of the PI3K/AKT signaling pathway and thereby suppresses proliferation. Therefore, in homeostasis, 

PTEN promotes chromosome stability and DNA repair (PMID: 24387334).Expression and function can be 

regulated by posttranslational modifications, including acetylation for decreased catalytic activity and 

ubiquitination for protein degradation, nuclear localization, and inhibition of phosphatase activity 

(PMID: 24656806).

What is the role of PTEN in cancer?

PTEN was first identified as a tumor suppressor when mutations or deletions were identified by two separate 

groups in a number of different cancer types including gliomas, breast, prostate, and kidney cancer 

(PMID: 9090379; PMID: 9072974). Non-inherited, or somatic, mutations in PTEN that lead to a loss of function 

in the protein have been shown to drive malignancy in tumors. PTEN hamartoma tumor syndrome (PHTS) is a 

group of inherited disorders that occur as a result of autosomal dominant mutations in the PTEN gene. These 

disorders include Cowden syndrome, Bannayan-Riley-Ruvalcaba syndrome, and Proteus syndrome. Because of 

the constitutive expression of PTEN, the symptoms are varied but often include neurodevelopmental issues and 

an increased risk of developing tumors, both benign and malignant (PMID: 26564076).

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; Myo9b mutations are associated with altered dendritic cell functions and increased susceptibility to autoimmune diabetes onset; Authors - Jing Zhang
human	WB	Biomater Res; High yield engineered nanovesicles from ADSC with enriched miR-21-5p promote angiogenesis in adipose tissue regeneration; Authors - Di Sun
human,mouse	IHC,WB	Drug Des Devel Ther; Network Pharmacology and Experimental Validation to Explore That Celastrol Targeting PTEN is the Potential Mechanism of Tripterygium wilfordii (Lév.) Hutch Against IgA Nephropathy; Authors - Juanyong Zhao
mouse	WB	J Pineal Res; Melatonin increases susceptibility to atrial fibrillation in obesity via Akt signaling impairment in response to lipid overload; Authors - Xinghua Qin
mouse	WB	Theranostics; Histone demethylase JMJD3 protects against renal fibrosis by suppressing TGFβ and Notch signaling and preserving PTEN expression.; Authors - Chao Yu
human	WB,IHC,IF	Cell Prolif; YTHDC1 positively regulates PTEN expression and plays a critical role in cisplatin resistance of bladder cancer; Authors - Yinjie Su

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