PTGER4 Polyklonaler Antikörper

PTGER4 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(15)

Kat-Nr. : 24895-1-AP

Synonyme

EP4R, EP4

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• 

  WB analysis of rat brain using 24895-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 24895-1-AP (PTGER4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 24895-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24895-1-AP (PTGER4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 24895-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 24895-1-AP (PTGER4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate hyperplasia using 24895-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 24895-1-AP (PTGER4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 24895-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 24895-1-AP (PTGER4 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human prostate hyperplasia using 24895-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 24895-1-AP (PTGER4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 24895-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 24895-1-AP (PTGER4 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhirngewebe, HL-60-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Prostatahyperplasie-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 5 publications below

Produktinformation

24895-1-AP bindet in WB, IHC, IF, ELISA PTGER4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PTGER4 fusion protein Ag19217
• Vollständiger Name: prostaglandin E receptor 4 (subtype EP4)
• Berechnetes Molekulargewicht: 488 aa, 53 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC101534
• Gene symbol: PTGER4
• Gene ID (NCBI): 5734
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PTGER4 (Prostaglandin E2 receptor EP4 subtype, also known as EP4R) is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.

Publikationen

Species	Application	Title
human	WB	J Clin Invest; Antibiotic pretreatment alleviates liver transplant damage in mice and humans.; Authors - Kojiro Nakamura
rat	WB	J Cell Mol Med; Inhibition of prostaglandin E2 receptor 4 by lnc000908 to promote the endothelial-mesenchymal transition participation in cardiac remodelling.; Authors - Xingxing Chen
rat	WB	Mol Pharm; PMID: 30109938; Authors - Huan Li
human	WB	Int J Mol Sci; Correlative Study on Impaired Prostaglandin E2 Regulation in Epicardial Adipose Tissue and its Role in Maladaptive Cardiac Remodeling via EPAC2 and ST2 Signaling in Overweight Cardiovascular Disease Subjects.; Authors - Elena Vianello
mouse	WB	Biomolecules; In Silico, In Vitro, and In Vivo Analysis of Tanshinone IIA and Cryptotanshinone from Salvia miltiorrhiza as Modulators of Cyclooxygenase-2/mPGES-1/Endothelial Prostaglandin EP3 Pathway.; Authors - Anella Saviano
	IF	Exp Cell Res; Prostaglandin E2 accelerated recovery of chemotherapy-induced intestinal damage by increasing expression of cyclin D.; Authors - Mengzhen Yue