PTGER4 Polyklonaler Antikörper

PTGER4 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 24895-1-AP

Synonyme

EP4, EP4R, PGE receptor EP4 subtype, PGE2 receptor EP4 subtype, Prostanoid EP4 receptor, PTGER2, PTGER4

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Galerie der Validierungsdaten

• 

  WB analysis of rat brain using 24895-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 24895-1-AP (PTGER4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 24895-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24895-1-AP (PTGER4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 24895-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 24895-1-AP (PTGER4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate hyperplasia using 24895-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 24895-1-AP (PTGER4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 24895-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 24895-1-AP (PTGER4 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human prostate hyperplasia using 24895-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 24895-1-AP (PTGER4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 24895-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 24895-1-AP (PTGER4 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhirngewebe, HL-60-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Prostatahyperplasie-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IF: See 2 publications below

Produktinformation

24895-1-AP bindet in WB, IHC, IF, ELISA PTGER4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PTGER4 fusion protein Ag19217
• Vollständiger Name: prostaglandin E receptor 4 (subtype EP4)
• Berechnetes Molekulargewicht: 488 aa, 53 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC101534
• Gene symbol: PTGER4
• Gene ID (NCBI): 5734
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Clin Invest; Antibiotic pretreatment alleviates liver transplant damage in mice and humans.; Authors - Kojiro Nakamura
rat	WB	J Cell Mol Med; Inhibition of prostaglandin E2 receptor 4 by lnc000908 to promote the endothelial-mesenchymal transition participation in cardiac remodelling.; Authors - Xingxing Chen
rat	WB	Mol Pharm; PMID: 30109938; Authors - Huan Li
human	WB	Int J Mol Sci; Correlative Study on Impaired Prostaglandin E2 Regulation in Epicardial Adipose Tissue and its Role in Maladaptive Cardiac Remodeling via EPAC2 and ST2 Signaling in Overweight Cardiovascular Disease Subjects.; Authors - Elena Vianello
mouse	WB	Biomolecules; In Silico, In Vitro, and In Vivo Analysis of Tanshinone IIA and Cryptotanshinone from Salvia miltiorrhiza as Modulators of Cyclooxygenase-2/mPGES-1/Endothelial Prostaglandin EP3 Pathway.; Authors - Anella Saviano
	IF	Exp Cell Res; Prostaglandin E2 accelerated recovery of chemotherapy-induced intestinal damage by increasing expression of cyclin D.; Authors - Mengzhen Yue

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