PTH1R Polyklonaler Antikörper

PTH1R Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29115-1-AP

Synonyme

Parathyroid hormone/parathyroid hormone-related peptide receptor, PFE, PTHR, PTHR1

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  WB analysis using 29115-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29115-1-AP (PTH1R antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 29115-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29115-1-AP (PTH1R antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 29115-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29115-1-AP (PTH1R antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 29115-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29115-1-AP (PTH1R antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 29115-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29115-1-AP (PTH1R antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 29115-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29115-1-AP (PTH1R antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Mausnierengewebe, Mauslebergewebe, Rattennierengewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

29115-1-AP bindet in WB, IHC, ELISA PTH1R und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PTH1R fusion protein Ag30762
• Vollständiger Name: parathyroid hormone 1 receptor
• Berechnetes Molekulargewicht: 593 aa, 66 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: BC110388
• Gene symbol: PTH1R
• Gene ID (NCBI): 5745
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PTH1R is a receptor for parathyroid hormone and parathyroid hormone-related peptide (PTHrP). PTH1R is a glycoprotein with seven transmembrane domains and it has an extracellular N-terminus region and an intracellular C-terminus region. The activity of PTH1R is mediated by G proteins which activate adenylyl cyclase. It has been reported that PTHrP and PTH1R are associated with the differentiation of bone and cartilage in the developing stages. PTH1R has several isoforms with 66/59 kDa and PTH1R can form a 120 kDa dimer in the non-reducing condition. With glycosylation modification, the molecular weight of PTH1R will be migrated to 90 kDa (PMID: 16783882).