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PTPIP51 Polyklonaler Antikörper
PTPIP51 Polyklonal Antikörper für WB, IHC, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human und mehr (3)
Anwendung
WB, IHC, IF, IP, COIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 20641-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | HEK-293-Zellen, Jurkat-Zellen | 
| Erfolgreiche IP | HEK-293-Zellen | 
| Erfolgreiche Detektion in IHC | human paracancerous of skin cancer,  humanes Zervixkarzinomgewebe,  humanes Hautgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. | 
Empfohlene Verdünnung
| Anwendung | Verdünnung | 
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 | 
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate | 
| Immunhistochemie (IHC) | IHC : 1:100-1:1200 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 3 publications below | 
| WB | See 15 publications below | 
| IHC | See 2 publications below | 
| IF | See 9 publications below | 
| CoIP | See 2 publications below | 
Produktinformation
20641-1-AP bindet in WB, IHC, IF, IP, COIP, ELISA PTPIP51 und zeigt Reaktivität mit human
| Getestete Reaktivität | human | 
| In Publikationen genannte Reaktivität | human, Affe, Maus, Ratte | 
| Wirt / Isotyp | Kaninchen / IgG | 
| Klonalität | Polyklonal | 
| Typ | Antikörper | 
| Immunogen | PTPIP51 fusion protein Ag14708 | 
| Vollständiger Name | family with sequence similarity 82, member A2 | 
| Berechnetes Molekulargewicht | 470 aa, 52 kDa | 
| Beobachtetes Molekulargewicht | 52-60 kDa | 
| GenBank-Zugangsnummer | BC008970 | 
| Gene symbol | PTPIP51 | 
| Gene ID (NCBI) | 55177 | 
| Konjugation | Unkonjugiert | 
| Form | Liquid | 
| Reinigungsmethode | Antigen-Affinitätsreinigung | 
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol | 
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. | 
Hintergrundinformationen
Protein tyrosine phosphatase-interacting protein 51 (PTPIP51),also known as RMDN3, is a mitochondrial membrane-anchored protein that interacts with ER membrane-bound VAPB. The VAPB-PTPIP51 interaction impacts on intracellular Ca2+ handling (PMID: 22131369, 28345618). PTPIP51 participates in multiple cellular processes, and dysfunction of PTPIP51 is implicated in diseases such as cancer and neurodegenerative disorders (PMID: 28345618). The known initiation sites in the Ptpip51 gene would lead to molecular protein masses of 52, 38 and 30 kDa (PMID: 18854601,19844996).
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for PTPIP51 antibody 20641-1-AP | Protokoll herunterladen | 
| IHC protocol for PTPIP51 antibody 20641-1-AP | Protokoll herunterladenl | 
| IP protocol for PTPIP51 antibody 20641-1-AP | Protokoll herunterladen | 
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen | 
Publikationen
| Species | Application | Title | 
|---|---|---|
| Nat Cell Biol MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.
 | ||
| Mol Cell The ER-Localized Transmembrane Protein EPG-3/VMP1 Regulates SERCA Activity to Control ER-Isolation Membrane Contacts for Autophagosome Formation. | ||
| J Cell Biol Atlastin 2/3 regulate ER targeting of the ULK1 complex to initiate autophagy. | ||
| EMBO Rep Nup358 restricts ER-mitochondria connectivity by modulating mTORC2/Akt/GSK3β signalling
 | ||
| J Cell Sci The role of Mfn2 in the structure and function of endoplasmic reticulum-mitochondrial tethering in vivo. | 
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
| FH Tom (Verified Customer) (03-23-2021) | Cos7 cells fixed in 4% PFA, perm in 0.1% Triton then blocked in 1% BSA. Mitochondria also labelled with TOMM20. Cells incubated with primary antibody (1/100) for 1 hour. 
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