PTPIP51 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PTPIP51 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IHC, IF, IP, COIP, ELISA

Konjugation

Unkonjugiert

Publikationen(22)

Rezensionen (1)

Kat-Nr. : 20641-1-AP

Synonyme

FAM82A2, Protein FAM82A2, hucep-10, hRMD-3, hRMD 3

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  WB analysis using 20641-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20641-1-AP (PTPIP51 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 20641-1-AP

  IP result of anti-PTPIP51 (IP:20641-1-AP, 4ug; Detection:20641-1-AP 1:1000) with HEK-293 cells lysate 1100ug.

• 

  IHC staining of human skin using 20641-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 20641-1-AP (PTPIP51 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 20641-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 20641-1-AP (PTPIP51 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human paracancerous of skin cancer using 20641-1-AP

  Immunohistochemical analysis of paraffin-embedded human paracancerous of skin cancer slide using 20641-1-AP (PTPIP51 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 20641-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20641-1-AP (PTPIP51 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervical cancer using 20641-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20641-1-AP (PTPIP51 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Jurkat-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: human paracancerous of skin cancer, humanes Zervixkarzinomgewebe, humanes Hautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 15 publications below
• IHC: See 2 publications below
• IF: See 9 publications below
• CoIP: See 2 publications below

Produktinformation

20641-1-AP bindet in WB, IHC, IF, IP, COIP, ELISA PTPIP51 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Affe, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PTPIP51 fusion protein Ag14708
• Vollständiger Name: family with sequence similarity 82, member A2
• Berechnetes Molekulargewicht: 470 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52-60 kDa
• GenBank-Zugangsnummer: BC008970
• Gene symbol: PTPIP51
• Gene ID (NCBI): 55177
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein tyrosine phosphatase-interacting protein 51 (PTPIP51),also known as RMDN3, is a mitochondrial membrane-anchored protein that interacts with ER membrane-bound VAPB. The VAPB-PTPIP51 interaction impacts on intracellular Ca2+ handling (PMID: 22131369, 28345618). PTPIP51 participates in multiple cellular processes, and dysfunction of PTPIP51 is implicated in diseases such as cancer and neurodegenerative disorders (PMID: 28345618). The known initiation sites in the Ptpip51 gene would lead to molecular protein masses of 52, 38 and 30 kDa (PMID: 18854601,19844996).

Publikationen

Species	Application	Title
human	WB	Cell Metab; A High-Density Human Mitochondrial Proximity Interaction Network.; Authors - Hana Antonicka
monkey	WB,IF	Nat Cell Biol; MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.; Authors - Tim König ; KD Validated
human	COIP	Mol Cell; The ER-Localized Transmembrane Protein EPG-3/VMP1 Regulates SERCA Activity to Control ER-Isolation Membrane Contacts for Autophagosome Formation.; Authors - Yan G Zhao
human	WB	J Cell Biol; Atlastin 2/3 regulate ER targeting of the ULK1 complex to initiate autophagy.; Authors - Nan Liu
human	WB,CoIP	EMBO Rep; Nup358 restricts ER-mitochondria connectivity by modulating mTORC2/Akt/GSK3β signalling; Authors - Misha Kalarikkal ; KD Validated
human	WB	J Cell Sci; The role of Mfn2 in the structure and function of endoplasmic reticulum-mitochondrial tethering in vivo.; Authors - Song Han

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tom (Verified Customer) (03-23-2021)

Cos7 cells fixed in 4% PFA, perm in 0.1% Triton then blocked in 1% BSA. Mitochondria also labelled with TOMM20. Cells incubated with primary antibody (1/100) for 1 hour.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: Cos7

PTPIP51 Antibody Immunofluorescence validation (1/100 dilution) in Cos7 (Cat no:20641-1-AP)