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PTPN9 Monoklonaler Antikörper

PTPN9 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D6E4

Kat-Nr. : CL488-67931

Synonyme

MEG2, PTPase MEG2, PTPMEG2, PTPN9

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CoraLite® Plus 488-conjugated PTPN9 Monoclonal ant (CL488-67931)

    IF Staining of U2OS using CL488-67931

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CoraLite® Plus 488 PTPN9 antibody (CL488-67931, Clone: 1D6E4 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CoraLite® Plus 488-conjugated PTPN9 Monoclonal ant (CL488-67931)

    IF Staining of U2OS using CL488-67931

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CoraLite® Plus 488 PTPN9 antibody (CL488-67931, Clone: 1D6E4 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite® Plus 488-conjugated PTPN9 Monoclonal ant (CL488-67931)

    FC experiment of MCF-7 using CL488-67931

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PTPN9 (CL488-67931, Clone:1D6E4) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFU2OS-Zellen
Erfolgreiche Detektion in FCMCF-7-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-67931 bindet in IF, FC (Intra) PTPN9 und zeigt Reaktivität mit Hausschwein, human, Maus

Getestete Reaktivität Hausschwein, human, Maus
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen PTPN9 fusion protein Ag30615
Vollständiger Name protein tyrosine phosphatase, non-receptor type 9
Berechnetes Molekulargewicht 593 aa, 68 kDa
Beobachtetes Molekulargewicht 68 kDa
GenBank-ZugangsnummerBC010863
Gene symbol PTPN9
Gene ID (NCBI) 5780
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PTPN9, Tyrosine-protein phosphatase non-receptor type 9, is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPN9 is involved in the transfer of hydrophobic ligands or in functions of the Golgi apparatus (PMID: 19167335). MiR-96 inhibits PTPN9 expression and consequently promotes proliferation, migration and invasion of breast cancer cells (PMID:27857177).

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 488 PTPN9 antibody CL488-67931Protokoll herunterladen
FC protocol for CL Plus 488 PTPN9 antibody CL488-67931Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen