IA-2/PTPRN Polyklonaler Antikörper

IA-2/PTPRN Polyklonal Antikörper für WB, IHC, IF-P, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 10584-1-AP

Synonyme

IA-2, PTPRN, ICA 512, ICA512-cleaved cytosolic fragment, ICA512-N-terminal fragment

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  WB analysis of mouse brain using 10584-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10584-1-AP (IA-2/PTPRN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10584-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10584-1-AP (IA-2/PTPRN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10584-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10584-1-AP (IA-2/PTPRN antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 10584-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10584-1-AP (IA-2/PTPRN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10584-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10584-1-AP (IA-2/PTPRN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 10584-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10584-1-AP (IA-2/PTPRN antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10584-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10584-1-AP (IA-2/PTPRN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10584-1-AP

  IP result of anti-IA-2/PTPRN (IP:10584-1-AP, 3ug; Detection:10584-1-AP 1:600) with mouse brain tissue lysate 7000ug.

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  IHC staining of rat pancreas using 10584-1-AP

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 10584-1-AP (IA-2/PTPRN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat pancreas using 10584-1-AP

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 10584-1-AP (IA-2/PTPRN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10584-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10584-1-AP (IA-2/PTPRN antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 10584-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10584-1-AP (IA-2/PTPRN antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of mouse pancreas using 10584-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using IA-2/PTPRN antibody (10584-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HEK-293 using 10584-1-AP

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human IA-2/PTPRN (10584-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HEK-293-Zellen, Mausnierengewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Ratten-Pankreasgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Pankreasgewebe
• Erfolgreiche Detektion in FC (Intra): HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 3 publications below

Produktinformation

10584-1-AP bindet in WB, IHC, IF-P, FC (Intra), IP, ELISA IA-2/PTPRN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IA-2/PTPRN fusion protein Ag0923
• Vollständiger Name: protein tyrosine phosphatase, receptor type, N
• Berechnetes Molekulargewicht: 105 kDa
• Beobachtetes Molekulargewicht: 60-65 kDa, 106-115 kDa
• GenBank-Zugangsnummer: BC007713
• Gene symbol: IA-2/PTPRN
• Gene ID (NCBI): 5798
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IA-2/PTPRN is a member of the protein tyrosine phosphatase (PTP) family, which contains a transmembrane region, an intracellular PTP-like domain and an extracellular N-terminus. Experiments found that IA-2/PTPRN localizes to secretory granules and is exclusively expressed in neuroendocrine cells (including pancreatic islets cell).(PMID: 10027571). IA-2/PTPRN was found to be a major autoantigen in insulin-dependent diabetes mellitus and the detection of autoantibodies against IA-2/PTPRN is commonly used as a diabetes diagnosis marker.

Publikationen

Species	Application	Title
human	WB,IHC	Front Cell Dev Biol; Overexpression of PTPRN Promotes Metastasis of Lung Adenocarcinoma and Suppresses NK Cell Cytotoxicity.; Authors - Xinyue Song
human	WB	J Biol Chem; Down-regulation of the islet-specific zinc transporter-8 (ZnT8) protects human insulinoma cells against inflammatory stress.; Authors - Chengfeng Merriman
		PLoS One; Sensitive detection of multiple islet autoantibodies in type 1 diabetes using small sample volumes by agglutination-PCR.; Authors - Felipe de Jesus Cortez
human	IHC	Cell Physiol Biochem; Prognostic Value of Phosphotyrosine Phosphatases in Hepatocellular Carcinoma.; Authors - Guangyan Zhangyuan
human	IHC	Transl Lung Cancer Res; Construction and validation of a prognostic model based on oxidative stress-related genes in non-small cell lung cancer (NSCLC): predicting patient outcomes and therapy responses; Authors - Dongfeng Sun