PTPRS Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PTPRS Polyklonal Antikörper für FC, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 13008-1-AP

Synonyme

PTPRS, PTPSIGMA, R PTP S, R PTP sigma, rPTPσ

---

Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 13008-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13008-1-AP (PTPRS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13008-1-AP

  WB result of PTPRS antibody (13008-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTPRS transfected HeLa cells.

• 

  WB analysis of mouse brain using 13008-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13008-1-AP (PTPRS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 13008-1-AP

  IP Result of anti-PTPRS (IP:13008-1-AP, 5ug; Detection:13008-1-AP 1:400) with mouse brain tissue lysate 6500ug.

• 

  IHC staining of human kidney using 13008-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13008-1-AP (PTPRS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 13008-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13008-1-AP (PTPRS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HeLa using 13008-1-AP

  1X10^6 HeLa cells were stained with 0.2ug PTPRS antibody (13008-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Maushirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

13008-1-AP bindet in WB, IP, IHC, IF, FC, ELISA PTPRS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PTPRS fusion protein Ag3691
• Vollständiger Name: protein tyrosine phosphatase, receptor type, S
• Berechnetes Molekulargewicht: 128aa,14 kDa; 1948aa,217 kDa
• Beobachtetes Molekulargewicht: 75-100 kDa
• GenBank-Zugangsnummer: BC029496
• Gene symbol: PTPRS
• Gene ID (NCBI): 5802
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Type II a receptor protein tyrosine phosphatases (rPTPσ) are cell surface receptors important for nervous system development, function, and repair.The expression of rPTPσ has previously been reported in b-cells and other target organs for INS although the probes chosen did not permit to distinguish between the splice variants.Proteolytic processing near the transmembrane domain generates an extracellular N-terminal E-domain of 130 kDa and a C-terminal P-domain of approximately 85 kDa of rPTPσ,and the short splice variants rPTPσ 3 and 4 contain an E-domain of 95 kDa (PMID: 16552719). rPTPσ expression was observed in tissue lysates of the adult mouse sensory-motor cortex and thoracic spinal cord (T8-T10) as a 75-80kDa immunoreactive band (PMID: 19780196).

Publikationen

Species	Application	Title
mouse	WB	Elife; Synaptic plasticity and cognitive function are disrupted in the absence of Lrp4.; Authors - Andrea M Gomez
human	WB	ACS Chem Neurosci; Small Molecules Targeting PTPσ-Trk Interactions Promote Sympathetic Nerve Regeneration.; Authors - Matthew R Blake
human	WB	J Gene Med; Downregulation of lncRNA HCG11 promotes cell proliferation of oral squamous cell carcinoma through sponging miR-455-5p.; Authors - Jingjing Wu
rat	WB,IHC	Curr Eye Res; Expression of Perineuronal Nets, Parvalbumin and Protein Tyrosine Phosphatase σ in the Rat Visual Cortex During Development and After BFD.; Authors - Liu Hui H
mouse	IF,WB	J Cell Biochem; Chondroitin sulfate proteoglycan represses neural stem/progenitor cells migration via PTPσ/α-actinin4 signaling pathway.; Authors - Jun Zhong