PTTG1IP Polyklonaler Antikörper

PTTG1IP Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 12575-1-AP

Synonyme

PTTG-binding factor, Pituitary tumor-transforming gene protein-binding factor, Pituitary tumor-transforming gene 1 protein-interacting protein, C21orf3, C21orf1

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  WB analysis using 12575-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12575-1-AP (PTTG1IP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 12575-1-AP

  IP result of anti-PTTG1IP (IP:12575-1-AP, 4ug; Detection:12575-1-AP 1:300) with HEK-293 cells lysate 2600 ug.

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  IHC staining of human testis using 12575-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12575-1-AP (PTTG1IP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 12575-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12575-1-AP (PTTG1IP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 12575-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12575-1-AP (PTTG1IP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 12575-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12575-1-AP (PTTG1IP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 12575-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 12575-1-AP (PTTG1IP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 12575-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 12575-1-AP (PTTG1IP antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 12575-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PTTG1IP antibody (12575-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of PC-3 using 12575-1-AP

  Immunofluorescent analysis of PC-3 cells using 12575-1-AP (PTTG1IP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Kolongewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

12575-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA PTTG1IP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PTTG1IP fusion protein Ag3250
• Vollständiger Name: pituitary tumor-transforming 1 interacting protein
• Berechnetes Molekulargewicht: 180 aa, 20 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC012858
• Gene symbol: PTTG1IP
• Gene ID (NCBI): 754
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Horm Metab Res; MicroRNA-524-5p Functions as a Tumor Suppressor in a Human Pituitary Tumor-Derived Cell Line.; Authors - Wang Zhen
human	IHC	Oncol Lett; Expression of proteins associated with epithelial-mesenchymal transition in esophageal squamous cell carcinoma.; Authors - Tian Zhang
human	WB	Pharmacol Res; PIAS3 promotes ferroptosis by regulating TXNIP via TGF-β signaling pathway in hepatocellular carcinoma; Authors - Wenfang Bao