PURB Polyklonaler Antikörper

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• KD/KO Validated

PURB Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, RIP, IP, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 18128-1-AP

Synonyme

PURB, PURBETA

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Galerie der Validierungsdaten

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  WB analysis using 18128-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18128-1-AP (PURB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 18128-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18128-1-AP (PURB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 18128-1-AP

  IP result of anti-PURB(IP:18128-1-AP, 4ug; Detection:18128-1-AP 1:3000) with HeLa cells lysate 1360 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C2C12-Zellen, HeLa-Zellen, Mauslebergewebe
• Erfolgreiche IP: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IF: See 2 publications below
• ChIP: See 2 publications below
• RIP: See 2 publications below

Produktinformation

18128-1-AP bindet in WB, RIP, IP, IF, ChIP, ELISA PURB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PURB fusion protein Ag12705
• Vollständiger Name: purine-rich element binding protein B
• Berechnetes Molekulargewicht: 312 aa, 33 kDa
• Beobachtetes Molekulargewicht: 33-40 kDa
• GenBank-Zugangsnummer: BC101735
• Gene symbol: PURB
• Gene ID (NCBI): 5814
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	RIP	Nat Commun; MaTAR25 lncRNA regulates the Tensin1 gene to impact breast cancer progression.; Authors - Kung-Chi Chang
mouse	WB	Mol Metab; Purβ promotes hepatic glucose production by increasing Adcy6 transcription.; Authors - Linna Jia
mouse	WB	Int J Mol Sci; Circular Intronic RNA circTTN Inhibits Host Gene Transcription and Myogenesis by Recruiting PURB Proteins to form Heterotypic Complexes; Authors - Nini Ai
bovine	IF,WB	J Cell Physiol; PURB is a positive regulator of amino acid-induced milk synthesis in bovine mammary epithelial cells.; Authors - Nan Huo
mouse	WB,ChIP	J Biol Chem; A novel Cbx1, PurB, and Sp3 complex mediates long-term silencing of tissue- and lineage-specific genes.; Authors - Syeda Samara Baksh
rat	WB,IF	Front Mol Neurosci; YAP and TAZ Regulate Cc2d1b and Purβ in Schwann Cells.; Authors - Belin Sophie ; KD Validated