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PYGM-Specific Polyklonaler Antikörper

PYGM-Specific Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Kat-Nr. : 19716-1-AP

Synonyme

PYGM, Myophosphorylase, EC:2.4.1.1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMausherzgewebe, rat heart
Erfolgreiche Detektion in IHChumanes Skelettmuskelgewebe, humanes Lebergewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:10000
Immunhistochemie (IHC)IHC : 1:20-1:200
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

19716-1-AP bindet in WB, IHC, IF/ICC, ELISA PYGM-Specific und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Hausschwein, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name phosphorylase, glycogen, muscle
Berechnetes Molekulargewicht 97 kDa
Beobachtetes Molekulargewicht 97-105 kDa
GenBank-ZugangsnummerNM_005609
Gene symbol PYGM
Gene ID (NCBI) 5837
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PYGM belongs to the glycogen phosphorylase family. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Defects in PYGM are the cause of glycogen storage disease type 5 (GSD5) which also known as McArdle disease. The antibody is specific to PYGM.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for PYGM-Specific antibody 19716-1-APProtokoll herunterladen
IHC protocol for PYGM-Specific antibody 19716-1-APProtokoll herunterladenl
IF protocol for PYGM-Specific antibody 19716-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Nature

Glycogen metabolism links glucose homeostasis to thermogenesis in adipocytes.

Authors - Omer Keinan
humanWB

Int J Biol Sci

PYGL-mediated glucose metabolism reprogramming promotes EMT phenotype and metastasis of pancreatic cancer

Authors - Qian Ji
mouseWB

Dis Model Mech

Absence of p.R50X Pygm read-through in McArdle disease cellular models.

Authors - Guillermo Tarrasó
mouseWB

Cells

Proteomic Landscape and Deduced Functions of the Cardiac 14-3-3 Protein Interactome

Authors - Jia-Hua Qu
humanWB

Oncol Lett

Analysis of the expression, function and signaling of glycogen phosphorylase isoforms in hepatocellular carcinoma.

Authors - Lingyu Jiang
humanWB

medRxiv

Identification of disease-specific extracellular vesicle-associated plasma protein biomarkers for Duchenne Muscular Dystrophy and Facioscapulohumeral Muscular Dystrophy

Authors - Mustafa Bilal Bayazit

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Patricia (Verified Customer) (02-25-2020)

Some non-specific bands appeared. Blocked in 5% skim milk and diluted antibody in TBS.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Ovarian cancer