Pan Acetylation Monoklonaler Antikörper

Pan Acetylation Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

chemical compound und mehr (5)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA, Acetylation assay

Konjugation

Unkonjugiert

Publikationen(52)

CloneNo.

1D5F2

Rezensionen (1)

Kat-Nr. : 66289-1-Ig

Synonyme

Pan Acetyl, Pan-Acetyl, Acetylation, Acetylation Lysine, Acetylated Lysine

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• 

  WB analysis of HeLa using 66289-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66289-1-Ig (Pan Acetylation Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 66289-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66289-1-Ig (Pan Acetylation antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of MDCK using 66289-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde) fixed MDCK cells using 66289-1-Ig (Pan Acetylation antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, MDCK-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 39 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• IP: See 3 publications below
• CoIP: See 5 publications below

Produktinformation

66289-1-Ig bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA, Acetylation assay Pan Acetylation und zeigt Reaktivität mit chemical compound

• Getestete Reaktivität: chemical compound
• In Publikationen genannte Reaktivität: human, drosophila, Maus, Ratte, Reis
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: Pan Acetylation
• Beobachtetes Molekulargewicht: 17 kDa
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Acetylation and De-acetylation are the important reaction in epigenetic. Many proteins, especially Histone, are with Acetylated of Lysine. This antibody is used to detect the Acetylation of Lysine. The 17 kDa in our detection is Acetylated Histone.

Publikationen

Species	Application	Title
mouse	WB,IF	Mol Cell; Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation; Authors - Chuanlong Wang
mouse	WB,IF	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li
		Sci Adv; Asparagine transporter supports macrophage inflammation via histone phosphorylation; Authors - Chuanlong Wang
Drosophila	WB	Dev Cell; HDAC6 Suppresses Age-Dependent Ectopic Fat Accumulation by Maintaining the Proteostasis of PLIN2 in Drosophila.; Authors - Yan Yan
human	WB	Cell Death Dis; RSL1D1 promotes the progression of colorectal cancer through RAN-mediated autophagy suppression.; Authors - Xunhua Liu
rice	Acetylation assay	Plant Biotechnol J; The rice RNase P protein subunit Rpp30 confers broad-spectrum resistance to fungal and bacterial pathogens.; Authors - Wei Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xiaoyu (Verified Customer) (07-18-2024)

Gives a nice, strong and specific signal in Western Blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T

Pan Acetylation Antibody Western Blot validation (1:1000 dilution) in 293T (Cat no:66289-1-Ig)