Phospho-DLK/MAP3K12 (Thr43) Polyklonaler Antikörper

Phospho-DLK/MAP3K12 (Thr43) Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 28277-1-AP

Synonyme

DLK, Leucine zipper protein kinase, MAP3K12, MAPK upstream kinase, Mixed lineage kinase, MUK, Phospho-DLK/MAP3K12 (Thr43), ZPK, ZPKP1

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  WB analysis using 28277-1-AP

  Non-treated PC-3 and PDGF (HZ-1215) treated PC-3 cells were subjected to SDS PAGE followed by western blot with 28277-1-AP (Phospho-DLK (Thr43) antibody) at dilution of 1:600 incubated at 4℃ overnight.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mit PDGF behandelte PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

28277-1-AP bindet in WB, ELISA Phospho-DLK/MAP3K12 (Thr43) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mitogen-activated protein kinase kinase kinase 12
• Berechnetes Molekulargewicht: 93 kda
• Beobachtetes Molekulargewicht: 93 kDa
• GenBank-Zugangsnummer: NM_001193511
• Gene symbol: MAP3K12
• Gene ID (NCBI): 7786
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAP3K12, also named as DLK ((dual leucine zipper kinase), is a part of a non-canonical MAPK signaling pathway. It has been reported that MAP3K12 can be activated by APOE through a receptor-mediated mechanism. MAP3K12 enhances the AP-1-mediated transcription of APP via a MAP kinase signal transduction pathway composed of MAP2K7, MAPK1/ERK2 and MAPK3/ERK1. MAP3K12 is required for pancreatic beta-cell proliferation during postnatal development and stress-induced JNK signaling in neurons.(PMID: 28111074, 25341110, 32189007)

Publikationen

Species	Application	Title
human	WB	Eur J Pharmacol; Comprehensive analysis of MAPK genes in the prognosis, immune characteristics, and drug treatment of renal clear cell carcinoma using bioinformatic analysis and Mendelian randomization; Authors - Xinyi Zheng ; KD Validated