Phospho-Chk1 (Ser345) Polyklonaler Antikörper

Phospho-Chk1 (Ser345) Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 28803-1-AP

Synonyme

CHEK1, CHK1, CHK1 checkpoint homolog, Chk 1, CHEK 1

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  WB analysis using 28803-1-AP

  Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 28803-1-AP (Phospho-Chk1 (Ser345) antibody) at dilution of 1:1000 incubated at room temperature for 1 hours.

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  IF Staining of HeLa using 28803-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Calyculin A treated HeLa cells using Phospho-Chk1 (Ser345) antibody (28803-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit Calyculin A behandelte NIH/3T3-Zellen, Mit Calyculin A behandelte HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: Mit Calyculin A behandelte HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below

Produktinformation

28803-1-AP bindet in WB, IF/ICC, ELISA Phospho-Chk1 (Ser345) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: CHK1 checkpoint homolog (S. pombe)
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC004202
• Gene symbol: Chk1
• Gene ID (NCBI): 1111
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The checkpoint kinase 1 (Chk1) is a conserved kinase that imposes cell cycle delays in response to impediments to DNA replication. Chk1 kinase is downstream of the ATR kinase. ATR phosphorylates Chk1 kinase and other proteins to ensure replication is being blocked as to avoid replication fork collapse and DNA damage. Activation of Chk1 involves phosphorylation at Ser317 and Ser345 by ATM/ATR, followed by autophosphorylation of Ser296. In vitro Chk1 binds to and phosphorylate the dual-specificity protein phosphatases Cdc25A, Cdc25B, and Cdc25C, which control cell cycle transitions by dephosphorylating cyclin-dependent kinases. (PMID:22941630, PMID: 32571801, PMID:19276361)

Publikationen

Species	Application	Title
human	WB	Transl Oncol; The ATR inhibitor VE-821 increases the sensitivity of gastric cancer cells to cisplatin; Authors - Haochen Su
human	WB	Phytomedicine; Epiberberine induced p53/p21-dependent G2/M cell cycle arrest and cell apoptosis in gastric cancer cells by activating γ-aminobutyric acid receptor- β3; Authors - Mengmeng Li
human	WB	J Exp Clin Cancer Res; HIF-1α-HPRT1 axis promotes tumorigenesis and gefitinib resistance by enhancing purine metabolism in EGFR-mutant lung adenocarcinoma; Authors - Pengyu Geng
human	WB	Cancer Sci; P2RY6 deletion promotes UVB-induced skin carcinogenesis by activating the PI3K/AKT signal pathway; Authors - Peng Xu
human	WB	Bioorg Chem; Structurally diverse tetrahydroxanthone analogues from Paraconiothyrium sp. AC31 with pyroptosis induction through targeted inhibition of PARP1 in hepatocellular carcinoma cells; Authors - Meng-Ke Zhang