Phospho-EPHA2 (Tyr588) Polyklonaler Antikörper

Phospho-EPHA2 (Tyr588) Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30263-1-AP

Synonyme

ECK, EPH receptor A2, EPHA2, Ephrin type A receptor 2, Epithelial cell kinase

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Galerie der Validierungsdaten

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  WB analysis using 30263-1-AP

  Non-treated and MG132 treated HepG2 cells were subjected to SDS PAGE followed by western blot with 30263-1-AP (Phospho-EPHA2 (Tyr588) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as the loading control.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MG132 treated HepG2 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30263-1-AP bindet in WB, ELISA Phospho-EPHA2 (Tyr588) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: EPH receptor A2
• Berechnetes Molekulargewicht: 976 aa, 108 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC037166
• Gene symbol: EPHA2
• Gene ID (NCBI): 1969
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ephrin type-A receptor 2 (EPHA2), belongs to the receptor tyrosine kinases (RTKs) family, that binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Research studies have shown that Eph receptors and ligands may be involved in many diseases including cancer (PMID: 11114742). The "reverse signaling" function, whereby the cytoplasmic domain becomes tyrosine phosphorylated, allows interactions with other proteins that may activate signaling pathways in the ligand-expressing cells. The detected molecular weight is around 110 kDa.