Phospho-PKC Alpha (Thr638) Polyklonaler Antikörper

Phospho-PKC Alpha (Thr638) Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 29123-1-AP

Synonyme

AAG6, Phospho-PKC Alpha (Thr638), PKC A, PKC alpha, PKCA, PRKACA, PRKCA, Protein kinase C alpha type, protein kinase C, alpha

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  WB analysis using 29123-1-AP

  Non-treated NIH/3T3, phosphatase inhibitor treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29123-1-AP (Phospho-PKC Alpha (Thr638) antibody) at dilution of 1:5000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  WB analysis using 29123-1-AP

  Non-treated and PMA treated HL-60 cells were subjected to SDS PAGE followed by western blot with 29123-1-AP (Phospho-PKC Alpha (Thr638) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 29123-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed λ phosphatase treated HeLa cells using Phospho-PKC Alpha (Thr638) antibody (29123-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mit λ-Phosphatase behandelte NIH/3T3-Zellen, PMA treated HL-60 cells
• Erfolgreiche Detektion in IF/ICC: Mit λ-Phosphatase behandelte HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below

Produktinformation

29123-1-AP bindet in WB, IF/ICC, ELISA Phospho-PKC Alpha (Thr638) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: protein kinase C, alpha
• Berechnetes Molekulargewicht: 77 kDa
• Beobachtetes Molekulargewicht: 77 kDa
• GenBank-Zugangsnummer: AK055431
• Gene symbol: PKC Alpha
• Gene ID (NCBI): 5578
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PKCs are a family of serine/threonine kinases involved in various processes in cells including proliferation, differentiation, cell survival, and apoptosis. PKC family is composed of three different subgroups: conventional (cPKC), the novel (nPKC) and atypical (aPKC). PKCα, β1, β2, and γ belong to cPKC, PKCδ, ε, η and θ are nPKC, whereas aPKC comprises of PKCζ and λ/ι. All PKCs consist of the N-terminal regulatory region and C-terminal catalytic region (kinase domain). PKCs are physiologically activated by various extracellular signals transduced by hormones, growth factors, cytokines or antigens. The presence of activated PKCs on internal membranes leads to the phosphorylation of various interacting proteins. (PMID: 32466765, PMID: 12417016)

Publikationen

Species	Application	Title
human	WB	Chem Biol Interact; Lead-induced cardiomyocytes apoptosis by inhibiting gap junction intercellular communication via modulating the PKCα/Cx43 signaling pathway; Authors - Qiong Wang
human	WB	Cancer Med; MAPK4 predicts poor prognosis and facilitates the proliferation and migration of glioma through the AKT/mTOR pathway; Authors - Jing Ren
mouse	WB	J Leukoc Biol; Transient receptor potential vanilloid 4 (TRPV4) in neutrophils enhances myocardial ischemia/reperfusion injury; Authors - Yuwei Wu
mouse	WB	Int J Mol Sci; Oryza sativa L. Indica Seed Coat Ameliorated Concanavalin A-Induced Acute Hepatitis in Mice via MDM2/p53 and PKCα/MAPK1 Signaling Pathways; Authors - Zhiye Zhao
human	WB	Cell Rep Med; Cholinergic signaling via muscarinic M1 receptor confers resistance to docetaxel in prostate cancer; Authors - Jing Wang
mouse	WB	Cytotechnology; β-sitosterol in Yijing Hugui decoction prevents cyclophosphamide-induced premature ovarian insufficiency via the AKT1/Nrf2 pathway; Authors - Li Chen