Phospho-S6 Ribosomal protein (Ser235/236) Polyklonaler Antikörper

Phospho-S6 Ribosomal protein (Ser235/236) Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Rezensionen (2)

Kat-Nr. : 29223-1-AP

Synonyme

p S6Ribosomal protein, p S6Ribosomal protein (Ser235/236), p S6Ribosomal protein Ser235/236, Phospho RPS6, Phospho RPS6 (Ser235/236)

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  WB analysis using 29223-1-AP

  Non-treated NIH/3T3, phosphatase inhibitor treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29223-1-AP (Phospho-S6 Ribosomal protein (Ser235/236) antibody) at dilution of 1:1000 incubated at room temperature for 1 hours.

• 

  WB analysis using 29223-1-AP

  Non-treated NIH/3T3 and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29223-1-AP (Phospho-S6 Ribosomal protein (Ser235/236) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mit PDGF behandelte NIH/3T3-Zellen, Mit λ-Phosphatase behandelte NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below
• IF: See 1 publications below

Produktinformation

29223-1-AP bindet in WB, IF, ELISA Phospho-S6 Ribosomal protein (Ser235/236) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ribosomal protein S6
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC000524
• Gene symbol: RPS6
• Gene ID (NCBI): 6194
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.

Publikationen

Species	Application	Title
Human	WB	Sci Rep; STK11 genetic alterations in metastatic EGFR mutant lung cancer; Authors - Dandan Yin
mouse	WB	ACS Omega; ROS-responsive MSC-derived Exosome Mimetics Carrying MHY1485 Alleviate Renal Ischemia Reperfusion Injury through Multiple Mechanisms; Authors - Zhiyu Qian
human	WB	Int J Med Sci; PRMT4 promotes hepatocellular carcinoma progression by activating AKT/mTOR signaling and indicates poor prognosis.; Authors - Peng Du
	WB	Metabolites; mTORC1 Mediates the Processes of Lysine Regulating Satellite Cells Proliferation, Apoptosis, and Autophagy; Authors - Mengqi Liu
human	WB	Aging (Albany NY); RIOK3 promotes mTORC1 activation by facilitating SLC7A2-mediated arginine uptake in pancreatic ductal adenocarcinoma; Authors - Henan Qin
mouse	IF	Exp Neurol; Synergistic effect of chemogenetic activation of corticospinal motoneurons and physical exercise in promoting functional recovery after spinal cord injury; Authors - Xueling Lin

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Morgane (Verified Customer) (09-15-2025)

Very good anti-phospho-antibody, very specific to RPS6

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HCT116

Phospho-S6 Ribosomal protein (Ser235/236) Antibody Western Blot validation (1/1000 dilution) in HCT116 (Cat no:29223-1-AP)

FH

Reyes (Verified Customer) (02-14-2025)

pS6 worked perfectly (in green) showing the mTOR activation in my neurons (in red NeuN) and Balloon Cells on human brain FFPE tissue.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

Phospho-S6 Ribosomal protein (Ser235/236) Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:29223-1-AP)