Phospho-SMAD1 (Ser195) Rekombinanter Antikörper

Phospho-SMAD1 (Ser195) Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

1K6

Kat-Nr. : 80257-1-RR

Synonyme

BSP 1, BSP1, hSMAD1, JV4 1, JV41, MAD homolog 1, Mad related protein 1, MADH1, MADR1, Mothers against DPP homolog 1, SMAD 1, SMAD family member 1, SMAD1

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Galerie der Validierungsdaten

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  WB analysis using 80257-1-RR

  Non-treated and UV treated A431 cells were subjected to SDS PAGE followed by western blot with 80257-1-RR (Phospho-SMAD1 (Ser195) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 80257-1-RR

  Non-treated and BMP2 (HZ-1128) treated HepG2 cells were subjected to SDS PAGE followed by western blot with 80257-1-RR (Phospho-SMAD1 (Ser195) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, Mit BMP2 behandelte HepG2-Zellen, mit UV behandelte A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

80257-1-RR bindet in WB, ELISA Phospho-SMAD1 (Ser195) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: SMAD family member 1
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC001878
• Gene symbol: SMAD1
• Gene ID (NCBI): 4086
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMAD family member 1 (Smad1) have been involved in metastatic progression of many cancer types. Smad1 can be induced by many tumor-stimulating cytokines such as the bone morphogenetic protein 2 (BMP2) and TNFα and plays important roles in cell invasion and metastasis. BMP2 signalling is initiated by binding to its specific receptors, which leads to in the phosphorylation and nuclear translocation of Smad1. Translocated Smad1 then modulates the expression of downstream osteogenic genes. (PMID: 32954678, PMID: 30008908)