Phospho-SPHK1 (Ser225) Polyklonaler Antikörper

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• KD/KO Validated

Phospho-SPHK1 (Ser225) Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 19561-1-AP

Synonyme

Phospho-SPHK1 (Ser225), SK 1, sphingosine kinase 1, SPHK, SPHK1, SPHK1 phopho, SPHK1-Phospho-Ser225, SPK, SPK 1

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Galerie der Validierungsdaten

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  WB analysis of L02 using 19561-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 19561-1-AP (SPHK1-Phospho-Ser225 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 19561-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 19561-1-AP (SPHK1-Phospho-Ser225 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 19561-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 19561-1-AP (SPHK1-Phospho-Ser225 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, humanes Hodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

19561-1-AP bindet in WB, IHC, IF, ELISA Phospho-SPHK1 (Ser225) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: sphingosine kinase 1
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 45-47 kDa
• GenBank-Zugangsnummer: NM_182965
• Gene symbol: SPHK1
• Gene ID (NCBI): 8877
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPHK1, also named as SPHK and SPK, catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. SPHK1 acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D, L-threo-dihydrosphingosine, N, N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. The antibody is specific to Ser(311)-phospho of SPHK1(isoform2) or Ser(225)-phospho of SPHK1(isoform1).

Publikationen

Species	Application	Title
human	IF	Cell Death Differ; Cell fate determined by the activation balance between PKR and SPHK1.; Authors - Han Qiao
human	WB	Ecotoxicol Environ Saf; Combined effect of co-exposure to di (2-ethylhexyl) phthalates and 50-Hz magnetic-fields on promoting human amniotic cells proliferation.; Authors - Liangjing Chen
human	WB	Ecotoxicol Environ Saf; A 50-Hz magnetic-field exposure promotes human amniotic cells proliferation via SphK-S1P-S1PR cascade mediated ERK signaling pathway.; Authors - Liangjing Chen
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Involvement of released sphingosine 1-phosphate/sphingosine 1-phosphate receptor axis in skeletal muscle atrophy.; Authors - Federica Pierucci
human,rat	WB,IHC	Phytomedicine; Geniposide alleviates VEGF-induced angiogenesis by inhibiting VEGFR2/PKC/ERK1/2-mediated SphK1 translocation.; Authors - Yan Wang
rat	WB	Phytother Res; Geniposide downregulates the VEGF/SphK1/S1P pathway and alleviates angiogenesis in rheumatoid arthritis in vivo and in vitro.; Authors - Yan Wang