Phospho-STAT1 (Ser727) Polyklonaler Antikörper
Phospho-STAT1 (Ser727) Polyklonal Antikörper für WB, IF/ICC, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human und mehr (1)
Anwendung
WB, IF/ICC, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 28977-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | mit IFN-gamma behandelte HeLa-Zellen |
| Erfolgreiche Detektion in IF/ICC | IFN alpha treated HeLa cells |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 15 publications below |
Produktinformation
28977-1-AP bindet in WB, IF/ICC, ELISA Phospho-STAT1 (Ser727) und zeigt Reaktivität mit human
| Getestete Reaktivität | human |
| In Publikationen genannte Reaktivität | human, Maus |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | Peptid |
| Vollständiger Name | signal transducer and activator of transcription 1, 91kDa |
| Berechnetes Molekulargewicht | 83 kDa |
| Beobachtetes Molekulargewicht | 84 kDa |
| GenBank-Zugangsnummer | BC002704 |
| Gene symbol | STAT1 |
| Gene ID (NCBI) | 6772 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
STAT1 (signal transducers and activators of transcription 1) is a member of the STAT protein family. STAT family members are phosphorylated by the receptor associated kinases, and then form homo-or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT1 activating phosphorylation at Ser727 is p38-dependant during lipotoxic stress. MLK3 activation triggers a MAPK signaling cascade resulting in STAT1 Ser727 phosphorylation. The phosphorylation of STAT1 Ser727 increases its nuclear localization, and transcriptional activity. (PMID: 28262979).
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for Phospho-STAT1 (Ser727) antibody 28977-1-AP | Protokoll herunterladen |
| IF protocol for Phospho-STAT1 (Ser727) antibody 28977-1-AP | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Gut Microbes Synergistic activity of Enterococcus Faecium-induced ferroptosis via expansion of IFN-γ+CD8+ T cell population in advanced hepatocellular carcinoma treated with sorafenib | ||
Acta Pharmacol Sin Celastrol inhibits the DPYSL2-JAK/STAT pathway by targeting mito-IDHs mediated mitochondrial metabolism to exhaust breast cancer | ||
Heliyon SMG9 is a novel prognostic-related biomarker in glioma correlating with ferroptosis and immune infiltrates | ||
J Asthma Sodium houttuyfonate Modulates the Lung Th1/Th2 Balance and Gut Microbiota to Protect Against Pathological Changes in Lung of Ovalbumin-induced Asthmatic mice | ||
J Virol Classical swine fever virus non-structural protein 4A recruits dihydroorotate dehydrogenase to facilitate viral replication | ||
Res Sq The RhoA GTPase regulates Type I Interferon Signaling in Systemic lupus erythematosus |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Emma (Verified Customer) (11-24-2022) | Works well for western blots. Only had a few unspecific bands but the antibody showed nice results for a phosphorylated protein.
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