Protein A Monoklonaler Antikörper

Protein A Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Staphylococcus aureus und mehr (1)

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

1G6A6

Kat-Nr. : 66945-1-Ig

Synonyme

Protein A

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Galerie der Validierungsdaten

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  WB analysis of Recombinant protein using 66945-1-Ig

  Commercial Protein A was deactivated and lysised followed by SDS-PAGE and then blot with 66945-1-Ig (Protein A antibody) at dilution of 1:10000 and isotype control antibody 66360-1-Ig at 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of ProteinA using 66945-1-Ig

  Commercial Protein A and negative samples (human plasma, HeLa cells, rat liver, mouse brain) were subjected to SDS-PAGE followed by western blot with 66945-1-Ig (Protein A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: recombinant protein

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IP: See 1 publications below

Produktinformation

66945-1-Ig bindet in WB, IP, ELISA Protein A und zeigt Reaktivität mit Staphylococcus aureus

• Getestete Reaktivität: Staphylococcus aureus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: Protein A
• GenBank-Zugangsnummer: EU695225
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region (PMID: 4163007). Engineered Protein A has been widely used in antibody purification due to its Fc binding property. However, trace of protein A might be leaked from purification resin thus causes contamination of final products. Antibody against Protein A or ELISA kits for Protein A could be used to evaluate leaking level of Protein A from resin. Protein A should be deactivated (e.g. boiling in 5-10% tween-20) before testing.

Publikationen

Species	Application	Title
	IP	Virus Res; HA2 from the Helicoverpa armigera nucleopolyhedrovirus: a WASP-related protein that activates Arp2/3-induced actin filament formation.; Authors - Qian Wang
human		J Biochem; Title: Cloaking the ACE2 receptor with salivary cationic proteins inhibits SARS-CoV-2 entry.; Authors - Katsutoshi Yoshizato