RAB11B-Specific Polyklonaler Antikörper

RAB11B-Specific Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 19742-1-AP

Synonyme

GTP binding protein YPT3, H YPT3, RAB11B, RAB11B-Specific, Ras related protein Rab 11B, YPT3

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Galerie der Validierungsdaten

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  WB analysis of human brain using 19742-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19742-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 19742-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 19742-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 19742-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 19742-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 19742-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 19742-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 19742-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19742-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 19742-1-AP

  IP result of anti-RAB11B-Specific (IP:19742-1-AP, 4ug; Detection:19742-1-AP 1:1000) with mouse brain tissue lysate 3200 ug.

• 

  IHC staining of human lung using 19742-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 19742-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 19742-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 19742-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 19742-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 19742-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, C6-Zellen, fetales humanes Hirngewebe, HEK-293-Zellen, HeLa-Zellen, humanes Hodengewebe, Maushodengewebe, PC-12-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Lungengewebe, humanes Hirngewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:300-1:600
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

19742-1-AP bindet in WB, IP, IHC, IF, ELISA RAB11B-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hund, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: RAB11B, member RAS oncogene family
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: NM_004218
• Gene symbol: RAB11B
• Gene ID (NCBI): 9230
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAB11B, also named as YPT3, belongs to the small GTPase superfamily and Rab family. RAB11B possesses GTPase activity. The antibody is specific to RAN11B. The antibody has no cross reaction to RAB11A.

Publikationen

Species	Application	Title
human	WB	Oncotarget; Upregulated long non-coding RNA AFAP1-AS1 expression is associated with progression and poor prognosis of nasopharyngeal carcinoma.; Authors - Hao Bo
human	WB	J Exp Clin Cancer Res; Long noncoding RNA AFAP1-AS1 acts as a competing endogenous RNA of miR-423-5p to facilitate nasopharyngeal carcinoma metastasis through regulating the Rho/Rac pathway.; Authors - Yu Lian
human	WB	Tumour Biol; AFAP1-AS1, a long noncoding RNA upregulated in lung cancer and promotes invasion and metastasis.; Authors - Zhaoyang Zeng
canine,rat	WB,IHC	J Histochem Cytochem; Canine Salivary Glands: Analysis of Rab and SNARE Protein Expression and SNARE Complex Formation With Diverse Tissue Properties.; Authors - Hiroshi Gomi
human	WB,IF	J Neurosci; The Late Endosomal Pathway Regulates the Ciliary Targeting of Tetraspanin Protein Peripherin 2.; Authors - Wataru Otsu