RAB22A Polyklonaler Antikörper

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• KD/KO Validated

RAB22A Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

22

Kat-Nr. : 12125-1-AP

Synonyme

Rab 22, RAB22, RAB22A, Ras related protein Rab 22A

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Galerie der Validierungsdaten

• 

  WB analysis of COLO 320 using 12125-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12125-1-AP (RAB22A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12125-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12125-1-AP (RAB22A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 12125-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12125-1-AP (RAB22A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 12125-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12125-1-AP (RAB22A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of COLO 320 using 12125-1-AP

  IP result of anti-RAB22A (IP:12125-1-AP, 4ug; Detection:12125-1-AP 1:300) with COLO 320 cells lysate 2560 ug.

• 

  IHC staining of human gliomas using 12125-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 12125-1-AP (RAB22A antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, A375-Zellen, BxPC-3-Zellen, Jurkat-Zellen
• Erfolgreiche IP: COLO 320-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 15 publications below
• IHC: See 7 publications below
• IF: See 6 publications below

Produktinformation

12125-1-AP bindet in WB, IP, IHC, IF, ELISA RAB22A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, hamster, Hund, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB22A fusion protein Ag2769
• Vollständiger Name: RAB22A, member RAS oncogene family
• Berechnetes Molekulargewicht: 194 aa, 22 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC015710
• Gene symbol: RAB22A
• Gene ID (NCBI): 57403
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAB22A is a small GTPase that is expressed ubiquitously in mammalian tissues. It localizes in the MHCI-containing recycling endosomal tubules and functions in endocytic pathway. RAB22A may regulate the dynamic interactions of endosomal compartments and it may be involved in the communication between the biosynthetic and early endocytic pathways. It shares 81% identity with RAB31.

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; An ARF6-Exportin-5 axis delivers pre-miRNA cargo to tumour microvesicles.; Authors - James W Clancy
human	WB,IHC	Clin Cancer Res; The Immune-microenvironment Confers Chemoresistance of Colorectal Cancer through Macrophage-derived IL-6.; Authors - Yuan Yin
human	WB,IF	EMBO Rep; Rab22A recruits BLOC-1 and BLOC-2 to promote the biogenesis of recycling endosomes.; Authors - Saurabh Shakya ; KD Validated
human	IF	J Cell Sci; A specific subset of RabGTPases controls cell surface exposure of MT1-MMP, extracellular matrix degradation and three-dimensional invasion of macrophages.; Authors - Wiesner Christiane C
mouse	WB	Traffic; Rab GTPases regulating phagosome maturation are differentially recruited to mycobacterial phagosomes.; Authors - Seto Shintaro S
hamster, mouse, human	WB,IF	Mol Biol Cell; Rabex-5 is a Rab22 effector and mediates a Rab22-Rab5 signaling cascade in endocytosis.; Authors - Zhu Huaiping H ; KD Validated

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