Rab23 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Rab23 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Rezensionen (1)

Kat-Nr. : 11101-1-AP

Synonyme

HSPC137, RAB23, Ras related protein Rab 23

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 11101-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11101-1-AP (RAB23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11101-1-AP

  WB result of RAB23 antibody (11101-1-AP, 1:3000) with si-Control and si-Rab23 transfected HEK293 cells.

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  WB analysis of human brain using 11101-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11101-1-AP (RAB23 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 11101-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11101-1-AP (RAB23 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11101-1-AP

  IP Result of anti-RAB23 (IP:11101-1-AP, 3ug; Detection:11101-1-AP 1:1000) with mouse brain tissue lysate 7000ug.

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  IHC staining of human liver cancer using 11101-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11101-1-AP (RAB23 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 11101-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11101-1-AP (RAB23 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HEK-293-Zellen, humanes Hirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 7 publications below
• WB: See 10 publications below
• IHC: See 6 publications below
• IF: See 4 publications below

Produktinformation

11101-1-AP bindet in WB, IP, IHC, IF, ELISA Rab23 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Rab23 fusion protein Ag1588
• Vollständiger Name: RAB23, member RAS oncogene family
• Berechnetes Molekulargewicht: 237 aa, 27 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC015021
• Gene symbol: RAB23
• Gene ID (NCBI): 51715
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rab23 is a novel member of large Rab small GTPase family. It plays important roles during embryonic development. The brain-enriched Rab23 is the only Rab protein that is known to have a distinct function during central nervous system development, playing an essential role as a negative regulator of the Sonic Hedgehog (Shh) pathway. RAB23 has recently been identified in mesangial cells (MCs), and could serve as a biomarker that indicates the severity of FSGS ( focal segmental glomerulosclerosis). Mutations in RAB23 may result in ACPS2 ( acrocephalopolysyndactyly type 2).

Publikationen

Species	Application	Title
human,mouse	WB,IF	Curr Biol; Planar Cell Polarity Effector Proteins Inturned and Fuzzy Form a Rab23 GEF Complex.; Authors - Andreas Gerondopoulos ; KD Validated
mouse	WB,IHC	Elife; RAB23 coordinates early osteogenesis by repressing FGF10-pERK1/2 and GLI1.; Authors - Md Rakibul Hasan
human	WB,IF	Oncotarget; Rab23 promotes squamous cell carcinoma cell migration and invasion via integrin β1/Rac1 pathway.; Authors - Qiang Jian ; KD Validated
human	WB	J Cell Sci; Differential role of Rab proteins in ciliary trafficking: Rab23 regulates smoothened levels.; Authors - Boehlke Christopher C ; KD Validated
human	WB,IHC	J Cancer Res Clin Oncol; MicroRNA-367-3p directly targets RAB23 and inhibits proliferation, migration and invasion of bladder cancer cells and increases cisplatin sensitivity; Authors - Xifeng Wei ; KD Validated
human,mouse	WB,IF	Exp Dermatol; Silencing of Rab23 by siRNA inhibits ultraviolet B-induced melanogenesis via downregulation of PKA/CREB/MITF.; Authors - Xuanjin Wei ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Guorong (Verified Customer) (11-22-2022)

Multiple bands distributed at 50 and 75 kDa were observed . Does not represent the performance in other kind of samples

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: ARPE-19

Rab23 Antibody Western Blot validation (1:500 dilution) in ARPE-19 (Cat no:11101-1-AP)