RAB25 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RAB25 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Rezensionen (1)

Kat-Nr. : 13189-1-AP

Synonyme

CATX 8, CATX8, CATX-8, Ras related protein Rab 25, Ras-related protein Rab-25

---

• 

  WB analysis of HT-29 using 13189-1-AP

  HT-29 cells were subjected to SDS PAGE followed by western blot with 13189-1-AP (RAB25 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Caco-2 using 13189-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 13189-1-AP (RAB25 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of T-47D using 13189-1-AP

  T-47D cells were subjected to SDS PAGE followed by western blot with 13189-1-AP (RAB25 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human renal cell carcinoma using 13189-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13189-1-AP (RAB25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 13189-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13189-1-AP (RAB25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 13189-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13189-1-AP (RAB25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 13189-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13189-1-AP (RAB25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-29-Zellen, Caco-2-Zellen, T-47D-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierenzellkarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

13189-1-AP bindet in WB, IHC, ELISA RAB25 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB25 fusion protein Ag3864
• Vollständiger Name: RAB25, member RAS oncogene family
• Berechnetes Molekulargewicht: 213 aa, 23 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC033322
• Gene symbol: RAB25
• Gene ID (NCBI): 57111
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	FEBS J; The protease ADAMTS5 controls ovarian cancer cell invasion, downstream of Rab25; Authors - Shengnan Yuan ; KD Validated
human	IHC	Transl Cancer Res; Rab25 acts as an oncogene and participates in the regulation of aerobic glycolysis via PKM2 in gastric adenocarcinoma; Authors - Tianyu Zheng

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Elena (Verified Customer) (05-13-2022)

The antibody detected a single band at the expected molecular weight, under both reducing and non-reducing conditions

• Applications: Western Blot
• Primary Antibody Dilution: 1:600
• Cell Tissue Type: Ovarian cancer cells