RAB8 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RAB8 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 11792-1-AP

Synonyme

RAB8, RAB8B, Ras related protein Rab 8B

---

Galerie der Validierungsdaten

• 

  WB analysis of fetal human brain using 11792-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 11792-1-AP (RAB8B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 11792-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 11792-1-AP (RAB8B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11792-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11792-1-AP (RAB8B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11792-1-AP

  IP Result of anti-RAB8B (IP:11792-1-AP, 4ug; Detection:11792-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human lung cancer using 11792-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11792-1-AP (RAB8B antibody) at dilution of 1:200 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of human lung cancer using 11792-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11792-1-AP (RAB8B antibody) at dilution of 1:200 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IF Staining of HepG2 using 11792-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 11792-1-AP (RAB8B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: fetales humanes Hirngewebe, humanes Hirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• FC: See 1 publications below

Produktinformation

11792-1-AP bindet in WB, IP, IHC, IF, FC, ELISA RAB8 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hund, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB8 fusion protein Ag2421
• Vollständiger Name: RAB8B, member RAS oncogene family
• Berechnetes Molekulargewicht: 207 aa, 24 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC020654
• Gene symbol: RAB8B
• Gene ID (NCBI): 51762
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Rab8 GTPase is a member of the Ras superfamily that functions in protein transport and membrane restructuring. RAB8 has two isoforms, RAB8A and RAB8B, that share 40% amino acid identity in the C-terminal variable region. RAB8 activity is regulated by specific guanine nucleotide exchange factors (GEFs), that mediate GTP loading, and GTPase activating proteins (GAPs) that convert them into the inactive GDP-bound form. RAB8 participates in recycling of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors to the dendritic spine surface as well as in the intracellular transport of the metabotropic glutamate receptor type 1 in neuronal cells. Rab8 is activated at the base of the cilium by its guanine nucleotide exchanger Rabin8. The antibody 11792-1-AP can detect both RAB8A and RAB8B. 55295-1-AP is specific to RAB8B and 55296-1-AP is specific to RAB8A.

Publikationen

Species	Application	Title
canine	WB	Nat Cell Biol; A molecular network for de novo generation of the apical surface and lumen.; Authors - Bryant David M DM ; KD Validated
human	WB	EMBO J; Loss of C9ORF72 impairs autophagy and synergizes with polyQ Ataxin-2 to induce motor neuron dysfunction and cell death.; Authors - Chantal Sellier
human	WB	Autophagy; TGFB1 is secreted through an unconventional pathway dependent on the autophagic machinery and cytoskeletal regulators.; Authors - Julian Nüchel ; KD Validated
mouse	IHC,IF	Hum Mol Genet; Rab8b GTPase, a protein transport regulator, is an interacting partner of otoferlin, defective in a human autosomal recessive deafness form.; Authors - Heidrych Paulina P
human	WB,IF	EMBO Rep; OCRL regulates lysosome positioning and mTORC1 activity through SSX2IP-mediated microtubule anchoring.; Authors - Biao Wang ; KD Validated
mouse	IF	Eur J Immunol; ACKR1 favors transcellular over paracellular T-cell diapedesis across the blood-brain barrier in neuroinflammation in vitro.; Authors - Luca Marchetti