RABEP1 Polyklonaler Antikörper

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• KD/KO Validated

RABEP1 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 14350-1-AP

Synonyme

Rabaptin-4, Rabaptin 5alpha, Rabaptin 5, Rabaptin 4, RAB5EP

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  WB analysis of HeLa using 14350-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14350-1-AP (RABEP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 14350-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 14350-1-AP (RABEP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 14350-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14350-1-AP (RABEP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14350-1-AP

  IP result of anti-RABEP1 (IP:14350-1-AP, 3ug; Detection:14350-1-AP 1:500) with HeLa cells lysate 3800ug.

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  IF Staining of HeLa using 14350-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14350-1-AP (RABEP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, humanes Lebergewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

14350-1-AP bindet in WB, IF/ICC, IP, ELISA RABEP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RABEP1 fusion protein Ag5693
• Vollständiger Name: rabaptin, RAB GTPase binding effector protein 1
• Berechnetes Molekulargewicht: 99 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC041700
• Gene symbol: RABEP1
• Gene ID (NCBI): 9135
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rab GTPase-binding effector protein 1 is an enzyme that in humans is encoded by the RABEP1 gene. It belongs to rabaptin protein family. RABEP1 act as linker between gamma-adaptin, RAB4A and RAB5A. It is involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. (PMID: 22841712)

Publikationen

Species	Application	Title
human	IF,IP	Elife; Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors.; Authors - Dawei Wang
Mouse		Stroke; Variants of Rab GTPase-Effector Binding Protein-2 Cause Variation in the Collateral Circulation and Severity of Stroke.; Authors - Jennifer L Lucitti
human	WB	J Cell Sci; His domain protein tyrosine phosphatase and Rabaptin-5 couple endo-lysosomal sorting of EGFR with endosomal maturation; Authors - Gabrielle Parkinson ; KD Validated
mouse	WB	J Mol Cell Cardiol; Decreased inspired oxygen stimulates de novo formation of coronary collaterals in adult heart.; Authors - Amir Aghajanian