RABEP2 Polyklonaler Antikörper

RABEP2 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 14625-1-AP

Synonyme

FRA, Rabaptin 5beta, RABEP2, RABPT5B

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 14625-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14625-1-AP (RABEP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 14625-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14625-1-AP (RABEP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14625-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14625-1-AP (RABEP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 14625-1-AP

  IP Result of anti-RABEP2 (IP:14625-1-AP, 4ug; Detection:14625-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human cervical cancer using 14625-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14625-1-AP (RABEP2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 14625-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14625-1-AP (RABEP2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 14625-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14625-1-AP (RABEP2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 14625-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14625-1-AP (RABEP2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 14625-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RABEP2 antibody (14625-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, Mauslebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IF: See 2 publications below

Produktinformation

14625-1-AP bindet in WB, IP, IHC, IF, ELISA RABEP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RABEP2 fusion protein Ag6197
• Vollständiger Name: rabaptin, RAB GTPase binding effector protein 2
• Berechnetes Molekulargewicht: 64 kDa
• Beobachtetes Molekulargewicht: 59-62 kDa
• GenBank-Zugangsnummer: BC058900
• Gene symbol: RABEP2
• Gene ID (NCBI): 79874
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RABEP2, also named as RABPT5B, plays a role in membrane trafficking and in homotypic early endosome fusion. It has two isoforms with MW 59-63 kDa.

Publikationen

Species	Application	Title
human	WB	Am J Hum Genet; A cross-species approach using an in vivo evaluation platform in mice demonstrates that sequence variation in human RABEP2 modulates ischemic stroke outcomes; Authors - Han Kyu Lee
mouse	WB	Stroke; Variants of Rab GTPase-Effector Binding Protein-2 Cause Variation in the Collateral Circulation and Severity of Stroke.; Authors - Jennifer L Lucitti
human	WB,IF	Sci Rep; Rab-GTPase binding effector protein 2 (RABEP2) is a primed substrate for Glycogen Synthase kinase-3 (GSK3).; Authors - Lisa Logie
mouse	WB	J Mol Cell Cardiol; Decreased inspired oxygen stimulates de novo formation of coronary collaterals in adult heart.; Authors - Amir Aghajanian
mouse	WB	J Hematol Oncol; LncRNAs H19 and HULC, activated by oxidative stress, promote cell migration and invasion in cholangiocarcinoma through a ceRNA manner.; Authors - Wen-Tao Wang
mouse	WB,IF	Arterioscler Thromb Vasc Biol; Hypoxia-Preconditioned Bone Marrow Mesenchymal Stem Cells Improved Cerebral Collateral Circulation and Stroke Outcome in Mice; Authors - Hao Tian