RABEPK/p40 Monoklonaler Antikörper

RABEPK/p40 Monoklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

1E11A3

Kat-Nr. : 66622-1-Ig

Synonyme

40 kDa Rab9 effector protein, p40, RAB9P40, RABEPK

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 66622-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66622-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66622-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66622-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66622-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 66622-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66622-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 66622-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 66622-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 66622-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293-Zellen, HeLa-Zellen, HSC-T6-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

66622-1-Ig bindet in WB, IHC, IF, ELISA RABEPK/p40 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RABEPK/p40 fusion protein Ag7796
• Vollständiger Name: Rab9 effector protein with kelch motifs
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC065725
• Gene symbol: RABEPK
• Gene ID (NCBI): 10244
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rab9 GTPase is required for the transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network in living cells, and in an in vitro system that reconstitutes this process. P40 is an effector of Rab9 that interacts preferentially with the active form of Rab9. p40 does not interact with Rab7 or K-Ras; it also fails to bind Rab9 when it is bound to GDI. The protein is found in cytosol, yet a significant fraction (~30%) is associated with cellular membranes. P40 is a very potent transport factor in that the pure, recombinant protein can stimulate, significantly, an in vitro transport assay that measures transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network.

Publikationen

Species	Application	Title
human	IHC	Exp Ther Med; Management of a rare ovarian carcinosarcoma: A case report and literature review; Authors - Jun Fu