RABL2B Rekombinanter Antikörper

RABL2B Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240501D3

Kat-Nr. : 83470-3-RR

Synonyme

Rab-like protein 2B, Rab like protein 2B, 240501D3

---

• 

  WB analysis using 83470-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83470-3-RR (RABL2B antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of A549 using 83470-3-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug RABL2B Recombinant antibody (83470-3-RR, Clone:240501D3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of MCF-7 using 83470-3-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug RABL2B Recombinant antibody (83470-3-RR, Clone:240501D3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 83470-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83470-3-RR against Human RABL2B were performed. The affinity constant is 0.256 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): A549-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83470-3-RR bindet in WB, FC (Intra), ELISA RABL2B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RABL2B fusion protein Ag2189
• Vollständiger Name: RAB, member of RAS oncogene family-like 2B
• Berechnetes Molekulargewicht: 229 aa, 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC024281
• Gene symbol: RABL2B
• Gene ID (NCBI): 11158
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rab-like protein 2B (RABL2B) is a member of a poorly characterised clade of the RAS GTPase superfamily, which plays an essential role in male fertility, sperm intraflagellar transport and tail assembly (PMID: 28553998, PMID: 28138870). A preferential expression of RABL2B in human tissues and lymphoblastoid cell lines was detected which is most pronounced in brain and placenta (PMID: 20138207).