RACK1; GNB2L1 Monoklonaler Antikörper

RACK1; GNB2L1 Monoklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

1E11A7

Kat-Nr. : 66940-1-Ig

Synonyme

Gnb2 rs1, GNB2L1, H12.3, HLC 7, PIG21, RACK1

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Galerie der Validierungsdaten

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  WB analysis using 66940-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66940-1-Ig (RACK1; GNB2L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 66940-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66940-1-Ig (RACK1; GNB2L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66940-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66940-1-Ig (RACK1; GNB2L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66940-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66940-1-Ig (RACK1; GNB2L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66940-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66940-1-Ig (RACK1; GNB2L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 66940-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66940-1-Ig (RACK1; GNB2L1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66940-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66940-1-Ig (RACK1; GNB2L1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 66940-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RACK1; GNB2L1 antibody (66940-1-Ig, Clone: 1E11A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Hausschwein-Lebergewebe, HepG2-Zellen, L02-Zellen, NIH/3T3-Zellen, T-47D-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

66940-1-Ig bindet in WB, IP, IHC, IF, CoIP, ELISA RACK1; GNB2L1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RACK1; GNB2L1 fusion protein Ag26708
• Vollständiger Name: guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC019093
• Gene symbol: GNB2L1
• Gene ID (NCBI): 10399
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. RACK1 (receptor for activated protein kinase C 1), encoded by GNB2L1 gene, is a 317 amino acid guanine nucleotide-binding protein subunit beta-2-like 1 protein which is involved in the recruitment, assembly and/or regulation of a variety of signaling molecules, it contains 7 WD-repeats and is implicated in various protein interaction activities. RACK1 is a component of the 40S ribosomal subunit involved in translational repression. Recent finding suggests that RACK1 may be a new promising diagnosis biomarker and therapeutic target for non-small-cell lung cancer (NSCLC).

Publikationen

Species	Application	Title
human	IF	J Clin Invest; CAP2 promotes gastric cancer metastasis by mediating the interaction between tumor cells and tumor-associated macrophages; Authors - Guohao Zhang
human	WB,IHC,IF,IP,CoIP	Cell Death Differ; Loss of SMURF2 expression enhances RACK1 stability and promotes ovarian cancer progression; Authors - Yanan Pi
mouse,rat	IP,WB	Am J Physiol Renal Physiol; Neuropilin 1 Promotes Unilateral Ureteral Obstruction-Induced Renal Fibrosis via RACK1 in Renal Tubular Epithelial Cells; Authors - Ling Hou