RALY Monoklonaler Antikörper

RALY Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B10C3

Kat-Nr. : CL488-68011

Synonyme

Autoantigen p542, hnRNP core protein C like 2, HNRPCL2, P542, RALY, RNA binding protein Raly

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Galerie der Validierungsdaten

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  IF Staining of A431 using CL488-68011

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 RALY antibody (CL488-68011, Clone: 2B10C3 ) at dilution of 1:600.

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  FC experiment of A431 using CL488-68011

  1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RALY (CL488-68011, Clone:2B10C3) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of A431 using CL488-68011

  1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RALY (CL488-68011, Clone:2B10C3) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: A431-Zellen
• Erfolgreiche Detektion in FC: A431-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:300-1:1200
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-68011 bindet in IF, FC (Intra) RALY und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RALY fusion protein Ag12250
• Vollständiger Name: RNA binding protein, autoantigenic (hnRNP-associated with lethal yellow homolog (mouse))
• Berechnetes Molekulargewicht: 307 aa, 33 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC103753
• Gene symbol: RALY
• Gene ID (NCBI): 22913
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNA-binding protein RALY is a protein that in humans is encoded by the RALY gene. In infectious mononucleosis, anti-EBNA-1 antibodies are produced which cross-react with multiple normal human proteins. The cross-reactivity is due to anti-gly/ala antibodies that cross-react with host proteins containing configurations like those in the EBNA-1 repeat. One such antigen is RALY which is a member of the heterogeneous nuclear ribonucleoprotein gene family.