RAP1A Rekombinanter Antikörper

RAP1A Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240438E10

Kat-Nr. : 83708-2-RR

Synonyme

Ras-related protein Krev-1, KREV1, GTP-binding protein smg p21A, G-22K, EC:3.6.5.2

---

• 

  WB analysis using 83708-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83708-2-RR (RAP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HeLa using 83708-2-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RAP1A Recombinant antibody (83708-2-RR, Clone:240438E10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 83708-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83708-2-RR against Human RAP1A were performed. The affinity constant is 0.232 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, A431-Zellen, HeLa-Zellen, humanes Thrombozytengewebe, K-562-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83708-2-RR bindet in WB, FC (Intra), ELISA RAP1A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RAP1A fusion protein Ag9488
• Vollständiger Name: RAP1A, member of RAS oncogene family
• Berechnetes Molekulargewicht: 184 aa, 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC014086
• Gene symbol: RAP1A
• Gene ID (NCBI): 5906
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAP1A(Ras-related protein Rap-1A), also known as KREV1. Induces morphological reversion of a cell line transformed by a Ras oncogene. The biological roles of RAP1A include inducing neurite outgrowth and embryonic angiogenesis(PMID: 17916086). As one member of RAS oncogene family, it is a widely expressed receptor,with high mRNA expression in many tissues, particularly abundant in the colon. In cells, RAP1A was localized in cytoplasm and cell membrane(PMID: 11359771, PMID: 11359771).