RASGRF1 Monoklonaler Antikörper

RASGRF1 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A4D4

Kat-Nr. : 67010-1-Ig

Synonyme

CDC25, CDC25L, GNRP, GRF1, GRF55, H GRF55, PP13187, Ras GRF1, RASGRF1

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Galerie der Validierungsdaten

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  WB analysis of pig cerebellum using 67010-1-Ig

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 67010-1-Ig (RASGRF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 67010-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67010-1-Ig (RASGRF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat cerebellum using 67010-1-Ig

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 67010-1-Ig (RASGRF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 67010-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67010-1-Ig (RASGRF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Cerebellum-Gewebe, Hausschwein-Hirngewebe, Ratten-Cerebellum-Gewebe, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67010-1-Ig bindet in WB, ELISA RASGRF1 und zeigt Reaktivität mit Hausschwein, Ratten

• Getestete Reaktivität: Hausschwein, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RASGRF1 fusion protein Ag6937
• Vollständiger Name: Ras protein-specific guanine nucleotide-releasing factor 1
• Berechnetes Molekulargewicht: 134 kDa
• Beobachtetes Molekulargewicht: 145 kDa
• GenBank-Zugangsnummer: BC040275
• Gene symbol: RASGRF1
• Gene ID (NCBI): 5923
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RasGRF1, a member of calmodulin-activated guanine-nucleotide exchange factors (GEFs), highly expressed at the synaptic junctions of the central nervous system (CNS). It is similar to the Saccharomyces cerevisiae CDC25. RASGRF1 serves as an in vivo activator for H-RAS and members of the R-RAS and RAC subfamilies. Several studies have shown that RasGRF1 also binds to microtubules and participate in the regulation of neurite outgrowth. RasGRF1 has been implicated in neuronal excitability. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory.