RB1CC1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RB1CC1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

92

Rezensionen (1)

Kat-Nr. : 17250-1-AP

Synonyme

CC1, DRAGOU14, FIP200, KIAA0203, RB1 inducible coiled coil 1, RB1CC1, RBICC

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 17250-1-AP

  WB result of RB1CC1 antibody (17250-1-AP, 1:2000) with si-Control and si-RB1CC1 transfected HEK293 cells.

• 

  WB analysis using 17250-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17250-1-AP (RB1CC1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17250-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17250-1-AP (RB1CC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 17250-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17250-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 17250-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17250-1-AP (RB1CC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 17250-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17250-1-AP (RB1CC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17250-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17250-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17250-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17250-1-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 17250-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17250-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 17250-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17250-1-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 17250-1-AP

  IP Result of anti-RB1CC1 (IP:17250-1-AP, 5ug; Detection:17250-1-AP 1:500) with HEK-293 cells lysate 2500ug.

• 

  IP experiment of HEK-293 using 17250-1-AP

  IP Result of anti-RB1CC1 (IP:17250-1-AP, 5ug; Detection:17250-1-AP 1:500) with HEK-293 cells lysate 1600ug.

• 

  IHC staining of human liver cancer using 17250-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17250-1-AP (RB1CC1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 17250-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17250-1-AP (RB1CC1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 17250-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17250-1-AP (RB1CC1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 17250-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17250-1-AP (RB1CC1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 17250-1-AP

  Immunofluorescent analysis of HepG2 cells, using RB1CC1 antibody 17250-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of MCF-7 using 17250-1-AP

  Immunofluorescent analysis of MCF-7 cells, using RB1CC1 antibody 17250-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 16 publications below
• WB: See 62 publications below
• IHC: See 4 publications below
• IF: See 31 publications below
• IP: See 1 publications below
• CoIP: See 3 publications below

Produktinformation

17250-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA RB1CC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RB1CC1 fusion protein Ag10508
• Vollständiger Name: RB1-inducible coiled-coil 1
• Berechnetes Molekulargewicht: 1594 aa, 183 kDa
• Beobachtetes Molekulargewicht: 200 kDa
• GenBank-Zugangsnummer: BC017556
• Gene symbol: RB1CC1
• Gene ID (NCBI): 9821
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RB1CC1, also named as RBICC or FIP200, is implicated in the regulation of RB1 expression and functions as a DNA-binding transcription factor. It is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation. Its expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. RB1CC1 is required for autophagosome formation. It is probably involved in the tumorigenesis of breast cancer. RB1CC1 is frequently mutated in breast cancer and shows characteristics of a classical tumor suppressor gene. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RB1CC1. the calculated molecular weight of RB1CC1 is 180 kDa, but the modified RB1CC1 is about 200 kDa.

Publikationen

Species	Application	Title
human	IF	Cell; Mammalian hybrid pre-autophagosomal structure HyPAS generates autophagosomes; Authors - Suresh Kumar
human	IF	Cell; CCT2 is an aggrephagy receptor for clearance of solid protein aggregates.; Authors - Xinyu Ma
mouse	WB	Nat Immunol; Essential role for GABARAP autophagy proteins in interferon-inducible GTPase-mediated host defense.; Authors - Miwa Sasai
human	WB,IF	Cell Res; A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis.; Authors - Shulin Li ; KD Validated
human	WB	Nat Cell Biol; The LC3-conjugation machinery specifies the loading of RNA-binding proteins into extracellular vesicles.; Authors - Andrew M Leidal ; KO Validated
human	IF	Mol Cell; TNIP1 inhibits selective autophagy via bipartite interaction with LC3/GABARAP and TAX1BP1; Authors - François Le Guerroué

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

SANGAPPA (Verified Customer) (01-04-2020)

Works very good in western blotting!

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Human endometrial stromal cells

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