RbAp46 Polyklonaler Antikörper

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• KD/KO Validated

RbAp46 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 20365-1-AP

Synonyme

RBBP7, Retinoblastoma-binding protein p46, Retinoblastoma-binding protein 7, RBBP-7, Nucleosome-remodeling factor subunit RBAP46

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  WB analysis using 20365-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20365-1-AP (RbAp46 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 20365-1-AP

  IP result of anti-RbAp46 (IP:20365-1-AP, 4ug; Detection:20365-1-AP 1:4000) with A431 cells lysate 2640 ug.

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  IHC staining of human cervical cancer using 20365-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20365-1-AP (RbAp46 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 20365-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20365-1-AP (RbAp46 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 20365-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20365-1-AP (RbAp46 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20365-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20365-1-AP (RbAp46 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20365-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20365-1-AP (RbAp46 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 20365-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20365-1-AP (RbAp46 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A431 using 20365-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using RbAp46 antibody (20365-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, COS-7-Zellen, HEK-293T-Zellen, HeLa-Zellen
• Erfolgreiche IP: A431-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 3 publications below

Produktinformation

20365-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA RbAp46 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: retinoblastoma binding protein 7
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: NM_002893
• Gene symbol: RBBP7
• Gene ID (NCBI): 5931
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBBP7, also named as RBAP46, belongs to the WD repeat RBAP46/RBAP48/MSI1 family. RBBP7 is a core histone-binding subunit that may target chromatin remodeling factors, histone acetyltransferases and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. It is a component of several complexes which regulate chromatin metabolism. The antibody is specific to RBBP7.

Publikationen

Species	Application	Title
human	WB	Cell Mol Gastroenterol Hepatol; Targeting HNRNPM Inhibits Cancer Stemness and Enhances Antitumor Immunity in Wnt-activated Hepatocellular Carcinoma.; Authors - Gui-Qi Zhu ; KD Validated
human	WB	Oncogene; HNF1B-mediated repression of SLUG is suppressed by EZH2 in aggressive prostate cancer.; Authors - Jianqing Wang
	IHC	Cancer Cell Int; Platelet-activating factor acetyl hydrolase IB2 dysregulated cell proliferation in ovarian cancer.; Authors - YingYing He
human	WB,IHC	Biochem Biophys Res Commun; PAFAH1B2 is a HIF1a target gene and promotes metastasis in pancreatic cancer.; Authors - Can Ma ; KD Validated
human	WB,IHC	Oncol Lett; RBBP7 is a prognostic biomarker in patients with esophageal squamous cell carcinoma.; Authors - Ning Yu ; KD Validated
human	WB	Oncogene; ZNF133 is a potent suppressor in breast carcinogenesis through dampening L1CAM, a driver for tumor progression; Authors - Lifang Li