RBM10 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RBM10 Polyklonal Antikörper für WB, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, CoIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Kat-Nr. : 14423-1-AP

Synonyme

DXS8237E, G patch domain-containing protein 9, GPATC9, GPATCH9, KIAA0122

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  WB analysis of human brain using 14423-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14423-1-AP (RBM10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14423-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14423-1-AP (RBM10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14423-1-AP

  IP result of anti-RBM10 (IP:14423-1-AP, 4ug; Detection:14423-1-AP 1:800) with mouse brain tissue lysate 4000ug.

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  IF Staining of mouse brain using 14423-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RBM10 antibody (14423-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 14423-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RBM10 antibody (14423-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 14423-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RBM10 antibody (14423-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IF-P: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 8 publications below
• WB: See 11 publications below
• IHC: See 4 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Produktinformation

14423-1-AP bindet in WB, IHC, IF-P, IP, CoIP, RIP, ELISA RBM10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RBM10 fusion protein Ag5788
• Vollständiger Name: RNA binding motif protein 10
• Berechnetes Molekulargewicht: 104 kDa
• Beobachtetes Molekulargewicht: 115 kDa
• GenBank-Zugangsnummer: BC024153
• Gene symbol: RBM10
• Gene ID (NCBI): 8241
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBM10, also named as RNA-binding protein 10, is a 930 amino acid protein, which is in the extranucleolar nucleoplasm constitutes hundreds of nuclear domains, which dynamically change their structures in a reversible manner. RBM10 may be involved in post-transcriptional processing, most probably in mRNA splicing. RBM10 has some isoforms with MV 100-130 kDa (PMID: 25889998 and PMID: 18315527) .

Publikationen

Species	Application	Title
human	WB,IP,IHC,IF	Proc Natl Acad Sci U S A; RNA-binding motif protein 10 inactivates c-Myc by partnering with ribosomal proteins uL18 and uL5; Authors - Hyemin Lee ; KD Validated
human	WB	Oncogene; RNA-binding motif protein 10 induces apoptosis and suppresses proliferation by activating p53.; Authors - Ji Hoon Jung ; KD Validated
human	WB,CoIP	Exp Cell Res; RBM10 regulates the tumorigenic potential of human cancer cells by modulating PPM1B and YBX1 activities; Authors - Yueyang Li ; KD Validated
human	WB	BMC Mol Biol; Splicing arrays reveal novel RBM10 targets, including SMN2 pre-mRNA.; Authors - Leslie C Sutherland ; KD Validated
human	WB	J Cell Mol Med; RBM10 inhibits cell proliferation of lung adenocarcinoma via RAP1/AKT/CREB signalling pathway.; Authors - Xin Jin
human	WB	Oxid Med Cell Longev; RBM10 Is a Biomarker Associated with Pan-Cancer Prognosis and Immune Infiltration: System Analysis Combined with In Vitro and Vivo Experiments; Authors - Yingyue Cao ; KD Validated