RBM10 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RBM10 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, RIP, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 14423-1-AP

Synonyme

DXS8237E, GPATC9, GPATCH9, KIAA0122, RBM10, RNA binding motif protein 10, RNA binding protein 10, RNA binding protein S1 1, S1 1, ZRANB5

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Galerie der Validierungsdaten

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  WB analysis of human brain using 14423-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14423-1-AP (RBM10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14423-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14423-1-AP (RBM10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14423-1-AP

  IP Result of anti-RBM10 (IP:14423-1-AP, 4ug; Detection:14423-1-AP 1:800) with mouse brain tissue lysate 4000ug.

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  IF Staining of mouse brain using 14423-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RBM10 antibody (14423-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 14423-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RBM10 antibody (14423-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 14423-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RBM10 antibody (14423-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IF: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• RIP: See 1 publications below

Produktinformation

14423-1-AP bindet in WB, RIP, IP, IHC, IF, ELISA RBM10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RBM10 fusion protein Ag5788
• Vollständiger Name: RNA binding motif protein 10
• Berechnetes Molekulargewicht: 104 kDa
• Beobachtetes Molekulargewicht: 115 kDa
• GenBank-Zugangsnummer: BC024153
• Gene symbol: RBM10
• Gene ID (NCBI): 8241
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBM10, also named as RNA-binding protein 10, is a 930 amino acid protein, which is in the extranucleolar nucleoplasm constitutes hundreds of nuclear domains, which dynamically change their structures in a reversible manner. RBM10 may be involved in post-transcriptional processing, most probably in mRNA splicing. RBM10 has some isoforms with MV 130 and 115 kDa (PMID: 25889998 and PMID: 18315527) .

Publikationen

Species	Application	Title
human	WB,IHC,IF,IP	Proc Natl Acad Sci U S A; RNA-binding motif protein 10 inactivates c-Myc by partnering with ribosomal proteins uL18 and uL5; Authors - Hyemin Lee ; KD Validated
human	WB	Oncogene; RNA-binding motif protein 10 induces apoptosis and suppresses proliferation by activating p53.; Authors - Ji Hoon Jung ; KD Validated
human	WB	BMC Mol Biol; Splicing arrays reveal novel RBM10 targets, including SMN2 pre-mRNA.; Authors - Leslie C Sutherland ; KD Validated
human	WB	J Cell Mol Med; RBM10 inhibits cell proliferation of lung adenocarcinoma via RAP1/AKT/CREB signalling pathway.; Authors - Xin Jin
human	WB,IHC,RIP	Cancer Cell Int; RBM10 regulates alternative splicing of lncRNA Neat1 to inhibit the invasion and metastasis of NSCLC; Authors - Shan Cong ; KD Validated
human	WB,IHC,IP	Life Sci; RBM10 recruits METTL3 to induce N6-methyladenosine-MALAT1-dependent modification, inhibiting the invasion and migration of NSCLC; Authors - Yingshu Cao ; KD Validated