RBM15 Monoklonaler Antikörper

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• KD/KO Validated

RBM15 Monoklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

4A1A4

Kat-Nr. : CL594-66059

Synonyme

RNA-binding motif protein 15, RBM 15, OTT1, OTT, One-twenty two protein 1

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  IF Staining of HeLa using CL594-66059

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 RBM15 antibody (CL594-66059, Clone: 4A1A4 ) at dilution of 1:200.

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  FC experiment of HeLa using CL594-66059

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human RBM15 (CL594-66059, Clone:4A1A4) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66059 bindet in IF/ICC, FC (Intra) RBM15 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RBM15 fusion protein Ag17970
• Vollständiger Name: RNA binding motif protein 15
• Berechnetes Molekulargewicht: 105 kDa
• Beobachtetes Molekulargewicht: 107 kDa
• GenBank-Zugangsnummer: BC006397
• Gene symbol: RBM15
• Gene ID (NCBI): 64783
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNA-binding motif protein 15 (RBM15) belongs to the SPEN protein family, which have repressor function in several signaling pathway, and may bind to RNA through interaction with spliceosome components. It was first described as a 5′ translocation partner of the MAL gene in t(1,22)(p13;q13) infant acute megakaryocytic leukemia, and it functions in murine hematopoiesis through modulating Notch-induced transcriptional activation, which play crucial roles in leukemogenesis. Decreasing RBM15 levels with RNA interference could inhibit the growth and proliferation, block the cell cycle, and induce apoptosis in CML cells.