RBM19 Polyklonaler Antikörper

RBM19 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19095-1-AP

Synonyme

KIAA0682, RBM19, RNA binding motif protein 19

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  WB analysis of mouse colon using 19095-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 19095-1-AP (RBM19 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 19095-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 19095-1-AP (RBM19 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 19095-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 19095-1-AP (RBM19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 19095-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 19095-1-AP (RBM19 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 19095-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 19095-1-AP (RBM19 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of MCF-7 using 19095-1-AP

  Immunofluorescent analysis of MCF-7 cells, using RBM19 antibody 19095-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Kolongewebe, COLO 320-Zellen, Maus-Dünndarmgewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

19095-1-AP bindet in WB, IHC, IF/ICC, ELISA RBM19 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RBM19 fusion protein Ag13545
• Vollständiger Name: RNA binding motif protein 19
• Berechnetes Molekulargewicht: 107 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC006137
• Gene symbol: RBM19
• Gene ID (NCBI): 9904
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBM19, also named as KIAA0682, is a 960 amino acid protein, which belongs to the RRM MRD1 family. RBM19 is expressed in the crypts of Lieberkuhn of the intestine and in intestinal neoplasia. RBM19 plays a role in embryo pre-implantation development. The calculated molecular weight of RBM19 is 107 kDa, but the modified RBM19 is about 120 kDa.