RBM25 Polyklonaler Antikörper

RBM25 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 25297-1-AP

Synonyme

Arg/Glu/Asp-rich protein of 120 kDa, NET52, Protein S164, RBM 25, RED120

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  WB analysis using 25297-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25297-1-AP (RBM25 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 25297-1-AP

  IP result of anti-RBM25 (IP:25297-1-AP, 4ug; Detection:25297-1-AP 1:1000) with HEK-293 cells lysate 1400 ug.

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  IHC staining of human colon cancer using 25297-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25297-1-AP (RBM25 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 25297-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM25 antibody (25297-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, Maushirngewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:150-1:600
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IP: See 2 publications below
• RIP: See 1 publications below

Produktinformation

25297-1-AP bindet in WB, IHC, IF/ICC, IP, RIP, ELISA RBM25 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RBM25 fusion protein Ag18244
• Vollständiger Name: RNA binding motif protein 25
• Berechnetes Molekulargewicht: 843 aa, 100 kDa
• Beobachtetes Molekulargewicht: 110-120 kDa
• GenBank-Zugangsnummer: BC113389
• Gene symbol: RBM25
• Gene ID (NCBI): 58517
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNA-binding protein 25 (RBM25), also named as Protein S164 or RNPC7, is a 843 amino acid protein, which contains one RRM domain and one PWI domain. RBM25 localizes in the nucleus and cytoplasm. RBM25 acts as a regulator of alternative pre-mRNA splicing and is involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression.

Publikationen

Species	Application	Title
human	WB,IP	Cell Insight; LUC7L3 is a downstream factor of SRSF1 and prevents genomic instability; Authors - Xiaqing Zhang
mouse	WB	Gene; ZC3H13 may participate in the ferroptosis process of sepsis-induced cardiomyopathy by regulating the expression of Pnn and Rbm25; Authors - Wenji Lin
human	WB,IHC	Sci China Life Sci; RBM25 depletion suppresses the growth of colon cancer cells through regulating alternative splicing of MNK2; Authors - Lili Zhi ; KD Validated
human	WB	Pathol Res Pract; RNA binding motif protein 25 is a negative prognostic biomarker and promotes cell proliferation via alternative splicing in hepatocellular carcinoma; Authors - Kai Xu
mouse	RIP	Arterioscler Thromb Vasc Biol; Super Enhancer-Associated Circular RNA-CircKrt4 Regulates Hypoxic Pulmonary Artery Endothelial Cell Dysfunction in Mice; Authors - Cui Ma
rat	IP	PeerJ; RBM25 binds to and regulates alternative splicing levels of Slc38a9, Csf1, and Coro6 to affect immune and inflammatory processes in H9c2 cells; Authors - Xin Tian