RBM3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RBM3 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, RIP, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

33

Kat-Nr. : 14363-1-AP

Synonyme

IS1 RNPL, Putative RNA binding protein 3, RBM3, RNA binding motif protein 3, RNPL

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Galerie der Validierungsdaten

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  WB analysis using 14363-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14363-1-AP (RBM3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14363-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14363-1-AP (RBM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 14363-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 14363-1-AP (RBM3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 14363-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14363-1-AP (RBM3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14363-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14363-1-AP (RBM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 14363-1-AP

  IP Result of anti-RBM3 (IP:14363-1-AP, 4ug; Detection:14363-1-AP 1:300) with MCF-7 cells lysate 3200ug.

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  IHC staining of human testis using 14363-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14363-1-AP (RBM3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 14363-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14363-1-AP (RBM3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 14363-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14363-1-AP (RBM3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 14363-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14363-1-AP (RBM3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 14363-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14363-1-AP (RBM3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 14363-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14363-1-AP (RBM3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 14363-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14363-1-AP (RBM3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 14363-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14363-1-AP (RBM3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 14363-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14363-1-AP (RBM3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 14363-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14363-1-AP (RBM3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 14363-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14363-1-AP (RBM3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A375-Zellen, HepG2-Zellen, Jurkat-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, Raji-Zellen
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Hirngewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 25 publications below
• IHC: See 7 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• RIP: See 2 publications below

Produktinformation

14363-1-AP bindet in WB, RIP, IP, IHC, IF, ELISA RBM3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Eichh?rnchen, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RBM3 fusion protein Ag5934
• Vollständiger Name: RNA binding motif (RNP1, RRM) protein 3
• Berechnetes Molekulargewicht: 17 kDa
• Beobachtetes Molekulargewicht: 17-20 kDa
• GenBank-Zugangsnummer: BC006825
• Gene symbol: RBM3
• Gene ID (NCBI): 5935
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBM3, also named as RNPL, is a cold-inducible mRNA binding protein that enhances global protein synthesis at both physiological and mild hypothermic temperatures. It reduces the relative abundance of microRNAs, when overexpressed. RBM3 enhances phosphoryaltion of translation initiation factors and active polysome formation. It is up-regulated in human tumors. RBM3 is a potential proto-oncogenic proteins upon overexpression. (PMID: 19900510 ).

Publikationen

Species	Application	Title
mouse	WB	Nature; RBM3 mediates structural plasticity and protective effects of cooling in neurodegeneration.; Authors - Diego Peretti ; KD Validated
human,squirrel	WB	Cell; iPSCs from a Hibernator Provide a Platform for Studying Cold Adaptation and Its Potential Medical Applications.; Authors - Jingxing Ou
		EBioMedicine; The RNA-binding protein RBM3 promotes cell proliferation in hepatocellular carcinoma by regulating circular RNA SCD-circRNA 2 production.; Authors - Wei Dong
human	WB	Clin Transl Med; LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing; Authors - Qi Wen Chen
mouse	WB	J Cell Biol; Cold-induced protein RBM3 orchestrates neurogenesis via modulating Yap mRNA stability in cold stress.; Authors - Wenlong Xia ; KD Validated
mouse	WB	Front Cell Dev Biol; The RNA-Binding Protein RBM3 Promotes Neural Stem Cell (NSC) Proliferation Under Hypoxia.; Authors - Jingyi Yan

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