RBM4 Polyklonaler Antikörper

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• KD/KO Validated

RBM4 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, RIP, PLA, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

17

Kat-Nr. : 11614-1-AP

Synonyme

hLark, LARK, Lark homolog, RBM4, RBM4A, RNA binding motif protein 4, RNA binding motif protein 4a, RNA binding protein 4, ZCCHC21, ZCRB3A

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 11614-1-AP

  WB result of RBM4 antibody (11614-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM4 transfected HeLa cells.

• 

  WB analysis of mouse brain using 11614-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11614-1-AP (RBM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11614-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11614-1-AP (RBM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11614-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11614-1-AP (RBM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11614-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11614-1-AP (RBM4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 11614-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11614-1-AP (RBM4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11614-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11614-1-AP (RBM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11614-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11614-1-AP (RBM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11614-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11614-1-AP (RBM4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 11614-1-AP

  IP Result of anti-RBM4 (IP:11614-1-AP, 3ug; Detection:11614-1-AP 1:1000) with mouse heart tissue lysate 4000ug.

• 

  IHC staining of human gliomas using 11614-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11614-1-AP (RBM4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 11614-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11614-1-AP (RBM4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 11614-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11614-1-AP (RBM4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11614-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11614-1-AP (RBM4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 11614-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11614-1-AP (RBM4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HeLa-Zellen, humanes Herzgewebe, humanes Hirngewebe, humanes Nierengewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Lungenkarzinomgewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 7 publications below
• WB: See 14 publications below
• IHC: See 5 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• RIP: See 1 publications below

Produktinformation

11614-1-AP bindet in WB, RIP, PLA, IP, IHC, IF, FC, ELISA RBM4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RBM4 fusion protein Ag2190
• Vollständiger Name: RNA binding motif protein 4
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC021120
• Gene symbol: RBM4
• Gene ID (NCBI): 5936
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBM4, RNA-binding motif protein4, contains 2 RRM-type RNA-binding motif and a retroviral-type(RT) zinc finger. RNA-binding factor participates in number of aspects of cellular processes such as alternative splicing of pre-mRNA and translation regulation. RBM4 recruits eIF4A1 to stimulate IRES-dependent translation in responds to cellular stress. Once assembled at the rHRE, the HIF2A-RBM4-eIF4E2 complex captures the 5-prime cap and targets mRNAs to polysomes for active translation, thereby evading hypoxia-induced repression of protein synthesis. This is a rabbit polyclonal antibody raised against the full-length of human RBM4

Publikationen

Species	Application	Title
human	WB	Nature; An oxygen-regulated switch in the protein synthesis machinery.; Authors - Uniacke James J
human	WB, IHC	Cancer Cell; The splicing factor RBM4 controls apoptosis, proliferation, and migration to suppress tumor progression.; Authors - Yang Wang ; KD Validated
human	WB	Nucleic Acids Res; Modulation of alternative splicing during early infection of human primary B lymphocytes with Epstein-Barr virus (EBV): a novel function for the viral EBNA-LP protein.; Authors - Evelyne Manet
human	WB	Nat Commun; A splicing isoform of TEAD4 attenuates the Hippo-YAP signalling to inhibit tumour proliferation.; Authors - Yangfan Qi
human	WB	Int J Mol Sci; G-Quadruplex Regulation of VEGFA mRNA Translation by RBM4.; Authors - Kangkang Niu ; KD Validated
human	IHC	Sci Rep; Prognostic value of decreased expression of RBM4 in human gastric cancer.; Authors - Hongmei Yong