RBM6 Polyklonaler Antikörper

RBM6 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 14360-1-AP

Synonyme

NY LU 12, Lung cancer antigen NY-LU-12, HLC 11, g16, DEF3

---

• 

  WB analysis using 14360-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14360-1-AP (RBM6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 14360-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14360-1-AP (RBM6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14360-1-AP

  IP result of anti-RBM6 (IP:14360-1-AP, 4ug; Detection:14360-1-AP 1:500) with HeLa cells lysate 2000ug.

• 

  IHC staining of human pancreas cancer using 14360-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14360-1-AP (RBM6 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 14360-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14360-1-AP (RBM6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Mausmilzgewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• RIP: See 1 publications below

Produktinformation

14360-1-AP bindet in WB, IHC, IF/ICC, IP, RIP, ELISA RBM6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RBM6 fusion protein Ag5743
• Vollständiger Name: RNA binding motif protein 6
• Berechnetes Molekulargewicht: 129 kDa
• Beobachtetes Molekulargewicht: 129 kDa
• GenBank-Zugangsnummer: BC046643
• Gene symbol: RBM6
• Gene ID (NCBI): 10180
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBM6, also named as DEF3, is a 1123 amino acid protein, which may interact with FAM168B. RBM6 is expressed ubiquitously in adults tissues. RBM6 specifically binds poly(G) RNA homopolymers in vitro. RBM6 exists some isoforms with MV 129 kDa, 69 kDa, and 59 kDa. onversely, fetal thymus and adult kidney express very little RBM6. In the hemopoietic system of mice, the expression of RBM6 is downregulated upon differentiation of progenitor cells into granulocytes but persists during macrophage development.

Publikationen

Species	Application	Title
human	IHC	Proteomics Clin Appl; RNA-Binding Motif Protein 6 is a Candidate Serum Biomarker for Pancreatic Cancer.; Authors - Baojun Duan
human	RIP	Front Biosci (Landmark Ed); Global RNA Interaction and Transcriptome Profiles Demonstrate the Potential Anti-Oncogenic Targets and Pathways of RBM6 in HeLa Cells; Authors - Ping Peng
human	WB	J Cell Mol Med; The Oncopromoting Gene RBM6 Inhibits Prostate Tumour Cell Migration During Epithelial-to-Mesenchymal Transition; Authors - Ruoyang Liu ; KD Validated