RBM8A,Y14 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RBM8A,Y14 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 14958-1-AP

Synonyme

Binder of OVCA1 1, BOV 1, BOV 1A, BOV 1B, BOV 1C, MDS014, RBM8, RBM8A, RBM8A,Y14, RBM8B, Ribonucleoprotein RBM8A, RNA binding motif protein 8A, RNA binding protein 8A, RNA binding protein Y14, Y14, ZNRP, ZRNP1

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Galerie der Validierungsdaten

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  WB analysis using 14958-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14958-1-AP

  WB result of RBM8A,Y14 antibody (14958-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM8A,Y14 transfected HeLa cells.

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  WB analysis using 14958-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14958-1-AP

  WB result of RBM8A,Y14 antibody (14958-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM8A,Y14 transfected HeLa cells.

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  WB analysis of Jurkat using 14958-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14958-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14958-1-AP (RBM8A,Y14 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  IP experiment of HeLa using 14958-1-AP

  IP Result of anti-RBM8A,Y14 (IP:14958-1-AP, 4ug; Detection:14958-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IHC staining of human brain using 14958-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 14958-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 14958-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 14958-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 14958-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 14958-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 14958-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 14958-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 14958-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 14958-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM8A,Y14 antibody (14958-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, Jurkat-Zellen, MDA-MB-453s-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Eierstockgewebe, humanes Hodengewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

14958-1-AP bindet in WB, IP, IHC, IF, ELISA RBM8A,Y14 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RBM8A,Y14 fusion protein Ag6800
• Vollständiger Name: RNA binding motif protein 8A
• Berechnetes Molekulargewicht: 20 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC017088
• Gene symbol: RBM8A
• Gene ID (NCBI): 9939
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cell Metab; NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.; Authors - Mi Kyung Park
mouse	IF	RNA; An atlas of chromatoid body components.; Authors - Oliver Meikar
human	WB,IHC	Front Oncol; RBM8A Promotes Glioblastoma Growth and Invasion Through the Notch/STAT3 Pathway.; Authors - Yan Lin ; KD Validated
human	WB	Cell Death Discov; CircEIF3H-IGF2BP2-HuR scaffold complex promotes TNBC progression via stabilizing HSPD1/RBM8A/G3BP1 mRNA.; Authors - Xiaojin Song