RBM8A,Y14 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RBM8A,Y14 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 14958-1-AP

Synonyme

RBM8A, BOV-1, BOV 1C, BOV 1B, BOV 1A

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  WB analysis using 14958-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 14958-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14958-1-AP

  WB result of RBM8A,Y14 antibody (14958-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM8A,Y14 transfected HeLa cells.

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  WB analysis of HeLa using 14958-1-AP

  WB result of RBM8A,Y14 antibody (14958-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM8A,Y14 transfected HeLa cells.

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  WB analysis of Jurkat using 14958-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14958-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14958-1-AP (RBM8A,Y14 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IP experiment of HeLa using 14958-1-AP

  IP result of anti-RBM8A,Y14 (IP:14958-1-AP, 4ug; Detection:14958-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IHC staining of human brain using 14958-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 14958-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 14958-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 14958-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 14958-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 14958-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 14958-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 14958-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HeLa using 14958-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM8A,Y14 antibody (14958-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 14958-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM8A,Y14 antibody (14958-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HeLa using 14958-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, Jurkat-Zellen, MDA-MB-453s-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Eierstockgewebe, humanes Milzgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

14958-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA RBM8A,Y14 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RBM8A,Y14 fusion protein Ag6800
• Vollständiger Name: RNA binding motif protein 8A
• Berechnetes Molekulargewicht: 20 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC017088
• Gene symbol: RBM8A
• Gene ID (NCBI): 9939
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cell Metab; NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.; Authors - Mi Kyung Park
mouse	IF	RNA; An atlas of chromatoid body components.; Authors - Oliver Meikar
human	WB,IHC	Front Oncol; RBM8A Promotes Glioblastoma Growth and Invasion Through the Notch/STAT3 Pathway.; Authors - Yan Lin ; KD Validated
human	WB	Cell Death Discov; CircEIF3H-IGF2BP2-HuR scaffold complex promotes TNBC progression via stabilizing HSPD1/RBM8A/G3BP1 mRNA.; Authors - Xiaojin Song
human	WB,IF	Cell Rep; The RNA-binding protein EIF4A3 promotes axon development by direct control of the cytoskeleton; Authors - Fernando C Alsina