RBP3 Polyklonaler Antikörper

RBP3 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 14352-1-AP

Synonyme

D10S64, D10S65, D10S66, IRBP, RBP3, RBPI, Retinol binding protein 3

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Galerie der Validierungsdaten

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  WB analysis of mouse eye using 14352-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 14352-1-AP (RBP3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 14352-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 14352-1-AP (RBP3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of Y79 using 14352-1-AP

  IP Result of anti-RBP3 (IP:14352-1-AP, 4ug; Detection:14352-1-AP 1:400) with Y79 cells lysate 2000ug.

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  IHC staining of human eye using 14352-1-AP

  Immunohistochemical analysis of paraffin-embedded human eye using 14352-1-AP (RBP3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human eye using 14352-1-AP

  Immunohistochemical analysis of paraffin-embedded human eye using 14352-1-AP (RBP3 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Augengewebe, Y79-Zellen
• Erfolgreiche IP: Y79-Zellen
• Erfolgreiche Detektion in IHC: humanes Augengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

14352-1-AP bindet in WB, IP, IHC, IF, ELISA RBP3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RBP3 fusion protein Ag5699
• Vollständiger Name: retinol binding protein 3, interstitial
• Berechnetes Molekulargewicht: 135 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC039844
• Gene symbol: RBP3
• Gene ID (NCBI): 5949
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBP3 (retinoid-binding protein 3), also called interstitial retinoid-binding protein (IRBP), is a large glycoprotein that is synthesized and secreted by rod photoreceptor cells into the interphotoreceptor matrix. It is known to bind retinoids and fatty acids. RBP3 migrates around 136-140 kDa in the western blot analysis. (PMID: 15928609)

Publikationen

Species	Application	Title
mouse	IP	J Exp Med; Identification of a novel cis-regulatory element essential for immune tolerance.; Authors - Taylor N LaFlam
human	IHC	Acta Biomater; Extracellular matrix component expression in human pluripotent stem cell-derived retinal organoids recapitulates retinogenesis in vivo and reveals an important role for IMPG1 and CD44 in the development of photoreceptors and interphotoreceptor matrix.; Authors - Majed Felemban
human	WB	Proc Natl Acad Sci U S A; Development of a therapeutic anti-HtrA1 antibody and the identification of DKK3 as a pharmacodynamic biomarker in geographic atrophy.; Authors - Irene Tom
mouse	WB	Diabetes; Interphotoreceptor Retinol-Binding Protein Ameliorates Diabetes-induced Retinal Dysfunction and Neurodegeneration through Rhodopsin.; Authors - Jianglei Chen
human	IF	iScience; A Genetic modification that reduces ON-bipolar cells in hESC-derived retinas enhances functional integration after transplantation.; Authors - Suguru Yamasaki