RBP4 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

RBP4 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1D12B11

Kat-Nr. : 66104-1-Ig

Synonyme

1D12B11, Plasma retinol binding protein, Plasma retinol-binding protein, Plasma retinol-binding protein(1-176), Plasma retinol-binding protein(1-179)

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  WB analysis of human plasma using 66104-1-Ig

  human blood were subjected to SDS PAGE followed by western blot with 66104-1-Ig (RBP4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66104-1-Ig

  WB result of RBP4 antibody (66104-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBP4 transfected HepG2 cells.

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  IHC staining of human liver cancer using 66104-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 66104-1-Ig (RBP4 Antibody) at dilution of 1:50.

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  IHC staining of human liver cancer using 66104-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 66104-1-Ig (RBP4 Antibody) at dilution of 1:50.

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  IHC staining of human liver using 66104-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66104-1-Ig (RBP4 Antibody) at dilution of 1:50.

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  IHC staining of human liver using 66104-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66104-1-Ig (RBP4 Antibody) at dilution of 1:50.

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  IF Staining of human liver cancer using 66104-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using RBP4 antibody (66104-1-Ig, Clone: 1D12B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 66104-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RBP4 antibody (66104-1-Ig, Clone: 1D12B11 ) at dilution of 1:3000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Human Blood, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Leberkarzinomgewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:1500-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66104-1-Ig bindet in WB, IHC, IF/ICC, IF-P, ELISA RBP4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Hausschwein
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RBP4 fusion protein Ag19295
• Vollständiger Name: retinol binding protein 4, plasma
• Berechnetes Molekulargewicht: 201 aa, 23 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC020633
• Gene symbol: RBP4
• Gene ID (NCBI): 5950
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBP4 (retinol-binding protein 4) is a carrier protein that transports vitamin A (retinol) from the liver to the peripheral tissues. Synthesized primarily by hepatocytes and adipocytes as a 21 kDa non-glycosylated protein, RBP4 is secreted into the circulation as a retinol-RBP4 complex. In plasma the RBP4-retinol complex is bound to transthyretin (TRR), which prevents prevent kidney filtration. Two truncated forms of RBP4, RBP4-L (truncated at Leu-183) and RBP4-LL (truncated at Leu-182 and Leu-183), exist by proteolytic process. RBP4-L and RBP4-LL, which do not bind TTR, are normally excreted into the urine but accumulate in the serum during renal failure. Urinary RBP4 has been reported as marker for glomerular disease. RBP4 also was identified as an adipokine that elevated in some INS-resistant states. Measurement of serum RBP4 could be used to assess the risk of INS resistance, type 2 diabetes, obesity, and cardiovascular disease. (18752671, 16034410)

Publikationen

Species	Application	Title
pig	WB	Commun Biol; Retinol binding protein 4 restricts PCV2 replication via selective autophagy degradation of viral ORF1 protein; Authors - Qingbing Han