RBPJ Monoklonaler Antikörper

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• KD/KO Validated

RBPJ Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

2F12G12

Kat-Nr. : 66132-1-Ig

Synonyme

CBF 1, CBF1, csl, IGKJRB, IGKJRB1, KBF2, NY-REN-30, RBP J, RBP J kappa, RBP JK, RBPJ, RBPJK, RBPSUH, SUH

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  WB analysis of HeLa using 66132-1-Ig

  WB result of RBPJ antibody (66132-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBPJ transfected HeLa cells.

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  WB analysis of HepG2 using 66132-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66132-1-Ig (RBPJ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 66132-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66132-1-Ig (RBPJ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, humanes Hirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IP: See 1 publications below

Produktinformation

66132-1-Ig bindet in WB, IP, ELISA RBPJ und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RBPJ fusion protein Ag6302
• Vollständiger Name: recombination signal binding protein for immunoglobulin kappa J region
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC064976
• Gene symbol: RBPJ
• Gene ID (NCBI): 3516
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Cell Mol Med; Identification and validation of basement membrane-associated gene AGRN as prognostic and immune-associated biomarkers in colorectal cancer patients; Authors - Jianrong Li
mouse	WB,IP	Mol Med; Celastrol promotes DNA damage and apoptosis in uterine corpus endometrial carcinoma via promotion of KAT2B-mediated RBPJ acetylation and repression of MCM4 transcription; Authors - Lipeng Pei