Calcipressin 1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Calcipressin 1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Rezensionen (1)

Kat-Nr. : 14869-1-AP

Synonyme

ADAPT78, Calcipressin 1, CSP1, DSC1, DSCR1, MCIP1, RCAN1, RCN1, regulator of calcineurin 1

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  WB analysis using 14869-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14869-1-AP (Calcipressin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 cells using 14869-1-AP

  WB result of RCAN1 antibody (14869-1-AP, 1:1000) with si-control and si-RCAN1 transfected SKOV-3 cells.

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  WB analysis of mouse skeletal muscle using 14869-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14869-1-AP (RCAN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 14869-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14869-1-AP (RCAN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 14869-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 14869-1-AP (RCAN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 14869-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 14869-1-AP (RCAN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 14869-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14869-1-AP (RCAN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 14869-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 14869-1-AP (RCAN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14869-1-AP

  IP result of anti-Calcipressin 1 (IP:14869-1-AP, 4ug; Detection:14869-1-AP 1:500) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human colon cancer using 14869-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14869-1-AP (RCAN1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 14869-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14869-1-AP (RCAN1 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, A431-Zellen, humanes Herzgewebe, Maus-Cerebellum-Gewebe, Mausherzgewebe, Maus-Skelettmuskelgewebe, SKOV-3-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

14869-1-AP bindet in WB, IHC, IP, ELISA Calcipressin 1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Calcipressin 1 fusion protein Ag6457
• Vollständiger Name: regulator of calcineurin 1
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 25-29 kDa
• GenBank-Zugangsnummer: BC002864
• Gene symbol: RCAN1
• Gene ID (NCBI): 1827
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Free Radic Biol Med; N6-methyladenosine modification of SPOP relieves ferroptosis and diabetic cardiomyopathy by enhancing ubiquitination of VDAC3; Authors - Wei Meng
human	WB	Front Med (Lausanne); The identification of hub-methylated differentially expressed genes in osteoarthritis patients is based on epigenomic and transcriptomic data; Authors - Zhen-Chen Chu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xu (Verified Customer) (10-06-2020)

It can produce a clear band in mouse brain with a stronger one in Ts65Dn mouse due to gene dose.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1500
• Cell Tissue Type: mouse brain

Calcipressin 1 Antibody Western Blot validation (1:1500 dilution) in mouse brain (Cat no:14869-1-AP)