RCL1 Polyklonaler Antikörper

RCL1 Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 15330-1-AP

Synonyme

RCL1, RNAC, RPC2, RPCL1, RTC2

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Galerie der Validierungsdaten

• 

  WB analysis of A431 using 15330-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 15330-1-AP (RCL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15330-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15330-1-AP (RCL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 15330-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 15330-1-AP (RCL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 15330-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 15330-1-AP (RCL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 15330-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15330-1-AP (RCL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 15330-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 15330-1-AP (RCL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A375 using 15330-1-AP

  Immunofluorescent analysis of A375 cells using 15330-1-AP (RCL1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, A375-Zellen, HepG2-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IF: A375-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 2 publications below

Produktinformation

15330-1-AP bindet in WB, IF, ELISA RCL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RCL1 fusion protein Ag7058
• Vollständiger Name: RNA terminal phosphate cyclase-like 1
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 41 kDa
• GenBank-Zugangsnummer: BC001025
• Gene symbol: RCL1
• Gene ID (NCBI): 10171
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IF	J Mol Cell Biol; Stability and function of RCL1 are dependent on interaction with BMS1; Authors - Yong Wang
human	WB,IF	Cancer Cell Int; Rcl1 suppresses tumor progression of hepatocellular carcinoma: a comprehensive analysis of bioinformatics and in vitro experiments.; Authors - Yu Jiaze